Figure 5

Validation of SA24T0 v SA72T0 differentially expressed genes. Summary of the quantitative RT-PCR (RT-qPCR) results for selected genes between the control quarters of animals infected with S. aureus for 24 hours (SA24T0) or 72 hours (SA72T0). The results are represented as the average mRNA level detected in SA24T0 (white bars) and SA72T0 (grey bars) relative to the sample with the lowest expression. The bars denote standard error of the mean, * and ** denote that the expression difference is statistically significant with P < 0.05 and P < 0.005 respectively. A. RT-qPCR results for six selected genes that were identified from the microarray data analysis as exhibiting differential expression between SA24T0 and SA72T0. B. RT-qPCR results comparing the differential expression of ABCG2, CXCL12, IDH1, TGFB1 and XDH between SA24T0 and SA72T0 samples.