Figure 1

Phylogenetic construction of the VKR family. A maximum likelihood tree was generated from the 40 VKR sequences using MEGA5 under the JTT+G+I model with 100 bootstrap repetitions. Outgroups are formed by insulin receptors (IR, outgroup 1), RTK-like orphan receptors (ROR, outgroup 2) and/or EGF receptors (EGFR, outgroup 3) of the following species: A. aegypti (AaIR, AAB17094.1), A. echiniator (AeEGFR, EGI67610.1), A. gambiae (AgIR, EAA00322.3), A. mellifera (AmROR, XP_397058.4), B. impatiens (BiROR, XP_003490221.1 ), Biomphalaria glabrata (BgIR, AAF31166.1), B. mori (BmIR, NP_001037011.1), C. floridanus (CfEGFR, EFN60989.1), Crassostrea gigas (CgIR, EKC21734.1; CgROR, EKC27495.1), C. sinensis (CsROR, GAA34401.2), C. teleta (CtIR, ELT96360.1), D. melanogaster (DmIR, AAC47458.1), D. plexippus (DplIR, EHJ65074.1), D. virilis (DvEgfr, ABD64816.1), E. multilocularis (EmEGFR, CAD56486.1), H. saltator (HsIR, EFN83767.1; HsEGFR, EFN75184.1) Hydra vulgaris (HvIR, Q25197.1), Ixodes scapularis (IsIR, XP_002416224.1), Lymnaea stagnalis (LsEGFR, ABQ10634.1), Metaseiulus occidentalis (MoIR, XP_003739590.1), N. vitripennis (NvROR, XP_001601308.2; NvEGFR, XP_001602830.2), P. humanus corporis (PhcIR, XP_002430961.1), S. mansoni (SmIR1, GenBank: AAN39120.1; SmIR2, GenBank: AAV65745.2 and SER, GenBank: AAA29866.1), S. purpuratus (SpIR, XP_784376.3; SpErbB4, XP_791361.3 and ROR_Sp, XP_003729469.1) and T. castaneum (TcIR, EFA11583.1). For VKR abbreviations and accession numbers, see Table 1.