Sleep is not just for the brain: transcriptional responses to sleep in peripheral tissues

BMC Genomics

Table 3 Significantly overrepresented functional annotations describing sleep-enhanced transcripts in the heart

	Cluster 1
Category	Term	P-Value	Fold Enrichment	FDR(%)
GOTERM_BP_FAT	GO:0044265 Cellular macromolecule catabolic process	1.1E-06	2.259	0.002
GOTERM_BP_FAT	GO:0019941 Modification-dependent protein catabolic process	3.8E-06	2.328	0.006
GOTERM_BP_FAT	GO:0051603 Proteolysis involved in cellular protein catabolic process	4.5E-06	2.278	0.008
GOTERM_BP_FAT	GO:0044257 Cellular protein catabolic process	5.2E-06	2.266	0.009
GOTERM_BP_FAT	GO:0009057 Macromolecule catabolic process	7.0E-06	2.102	0.012
GOTERM_BP_FAT	GO:0030163 Protein catabolic process	1.1E-05	2.188	0.019
SP_PIR_KEYWORDS	Ublquitin conjugation pathway	1.9E-05	2.242	0.025
	Cluster 2
Category	Term	P-Value	Fold Enrichment	FDR(%)
SP_PIR_KEYWORDS	Zinc-finger	8.5E-04	1.543	1.147
SP_PIR_KEYWORDS	Zinc	1.5E-03	1.393	2.007
SP_PIR_KEYWORDS	Metal-binding	2.9E-03	1.293	3.845
	Cluster 3
Category	Term	P-Value	Fold Enrichment	FDR(%)
GOTERM_BP_FAT	GO:0006259 DNA metabolic process	3.0E-05	2.360	0.050
GOTERM_BP_FAT	GO:0006281 DNA repair	2.2E-04	2.732	0.363
GOTERM_BP_FAT	GO:0006974 Response to DNA damage stimulus	2.8E-04	2.444	0.463
SP_PIR_KEYWORDS	DNA damage	5.5E-04	2.720	0.743
SP_PIR_KEYWORDS	DNA repair	1.4E-03	2.693	1.914
	Cluster 4
Category	Term	P-Value	Fold Enrichment	FDR(%)
SP_PIR_KEYWORDS	RNA-binding	1.4E-07	2.568	2E-04
KEGG_PATHWAY	Spliceosome	7.3E-05	3.757	9E-02

Sleep enhanced transcripts demonstrate both significantly increased expression in sleep as compared to sleep deprivation (FDR < 1%) and increasing expression as spontaneous sleep progresses (FDR <1%). Overrepresented functional annotations describing this group of transcripts that met a FDR cutoff of 5% are shown. Clusters describe overlapping gene annotations as determined by the DAVID functional clustering feature.

ISSN: 1471-2164