Figure 4

PCR markers for six OPR and LOX genes, TaOPR1-A, TaOPR2-A, TaLOX1-A, TaOPR7-B, TaLOX6-B, and TaLOX2-B . A-F) Lanes: 1 undigested Jagger 2 undigested 2174 3 digested Jagger 4 digested 2174. M Molecular marker. A) TaOPR1-A marker. Digestion with KpnI shows polymorphic band patterns for Jagger (213 bp + 124 bp) and 2174 (36 bp + 177 bp + 124 bp) (the 36 bp band was run out of the gel). B) TaOPR2-A marker. Digestion with HincII shows polymorphic band patterns for Jagger (20 bp + 321 bp + 292 bp) and 2174 (342 bp + 215 bp + 77 bp) (the 20 bp and 77 bp bands were run out of the gel). C) TaLOX1-A marker. Digestion with ScrFI shows polymorphic band patterns for Jagger and 2174, in which 2174 has an extra ~250 bp fragment. D) TaOPR7-B marker. Digestion with DdeI shows polymorphic band patterns for Jagger (487 bp) and 2174 (35 bp + 452 bp) (the 35 bp band was run out of the gel). E) TaLOX6-B marker. PCR products show polymorphic band patterns for Jagger and 2174 with 8-bp indels. F) TaLOX2-B marker. PCR products show polymorphic band patterns for Jagger and 2174 with an extra lower band in Jagger. PCR products were separated in a 2% agarose gel (A-D and F) or 6% acrylamide gel (E).