Figure 9

Comparative digestion of the PCR amplified horse butyrylcholinesterase gene with frequently cleaving REases. TaqII affinity star cleaved 1 μg horse butyrylcholinesterase gene DNA (1841 bp) was electrophoresed on 1.5% agarose/TBE gel. Cleavage was carried out at 65°C for 16 h with 5 μg (40 pmol) of enzyme in 50 μl of reaction volume. Lane M1, Fermentas 1 kb DNA Ladder (selected bands marked); lane M2, Fermentas 100 bp DNA Ladder (selected bands marked); Lane K, undigested DNA; lanes 1–4, DNA digested with TaqII (Methods): lane 1, without SIN and DMSO; lane 2, with SIN, no DMSO; lane 3, no SIN, 20% DMSO; lane 4, with SIN, 20% DMSO; lane 5, DNA digested with HaeIII (5 units); lane 6, DNA digested with CviJI (0.25 unit).