Skip to main content

Table 5 Growth conditions for RNA-seq

From: The genome and transcriptome of the pine saprophyte Ophiostoma piceae, and a comparison with the bark beetle-associated pine pathogen Grosmannia clavigera

Medium

Carbon sources or treatment

Duration

CM

No treatment

14 h

CM

No treatment

40 h

CM

200 μl Terpene blend

14 h

CM

200 μl Terpene blend

40 h

YNB

Mannose (1% w/v)

5 days

YNB

*TG: Olive Oil (1% v/v)

5 days

YNB

Oleic acid (0.5% v/v)

5 days

Sawdust

No treatment

1 weeks

  1. * Triglyceride: Olive oil 80% + Fatty acids 20%.
  2. All treatment times were calculated from the initial transfer of actively growing hyphae onto the appropriate medium. CM = 0.17% yeast nitrogen base without amino acids, 1.5% granulated agar, 1% maltose, 0.1% potassium hydrogen phthalate, 0.3% asparagine. YNB = 0.67% yeast nitrogen base without amino acid and 1.5% agar with carbon sources indicated as treatment. The terpene blend was a mixture of monoterpenes and diterpenes as described by Lah et al. [35].