Figure 2

Lineage relationships inferred from methods based on genetic distance. (A) Modified eBURST analysis, showing “population snapshot” of single cell clones in mouse 1. Clusters of related single cell clones and individual unlinked clones are displayed as a single modified eBURST diagram by using the distance value D=0.2 as cut-off. Clusters of linked single cell clones correspond to complexes that share highly similar mutational profiles. Each single cell clone is represented as a dot with color indicative of its tissue origin. (Mouse 2 shown in Figure S1.) (B) Network representation depicting mutational similarities among single cell clones between both mice. Significant similarities between single cell clones for mouse 1 are shown with grey connecting lines. Each single cell clone is depicted as a dot with different colors indicative of tissue origin while the layout on the graph reflects relative anatomical location on the anteroposterior axis. The diameter of the circles correlates with the average distance within tissues. Orange lines show relationships that are conserved in mouse 2. (C) Scatter plot of distance between equivalent pairs of tissue, comparing mouse 1 to mouse 2. Distances of specific tissues to the zygote are colored orange; a trend line indicates their correlation. Among these comparisons, the distances between individual tissues to the zygote are largely conserved between the two mice.