Figure 2

Megalin expression is uniform whereas amnionless accumulates in cubilin-deficient renal proximal tubules of Cubn^{+/del exon 1–6;EGFP}mice. A-C show confocal images of a section of Cubn^{+/del exon 1–6;EGFP} mouse kidney labeled with anti-megalin IgG (A), anti-cubilin (B). C is a merged image of anti-megalin and anti-cubilin immunolabeling together with Draq5 nuclear staining (blue). Boxed areas in A and B highlight an example of a proximal tubule displaying strong anti-megalin immunolabeling and relatively weak anti-cubilin labeling. D shows a confocal image from a section of a Cubn^{+/del exon 1–6;EGFP} mouse kidney labeled with anti-amnionless (Amn). E is a merged image of the section of Cubn^{+/del exon 1–6;EGFP} mouse kidney shown in D labeled with anti-amnionless, anti-cubilin and the nuclear stain, Draq5 (blue). F is a digitally zoomed in view of the boxed areas in D and E showing a proximal tubule displaying strong anti-cubilin immunolabeling adjacent to two proximal tubules with little or no anti-cubilin labeling. Arrowheads indicate intracellular accumulations of Amn immunolabeling. G, glomerulus.