Figure 2

Gepard dotplot alignments of 247 mycobacteriophages using TMP nucleotide or amino acid sequences recover assigned subcluster relationships with an accuracy of 97.6±1.92%. Gepard dotplots were constructed using the nucleotide (A) or amino acid sequence (B) of TMP to determine the frequency of recovering the cluster assigned by whole genome analysis. Cluster assignment for the 247 mycobacteriophages are available at http://www.phagesdb.org and are indicated on the axes. Sequences are plotted in the following cluster order A1, A2, A3, A4, A5, A6, A7, A8, A9, A10, B1, B2, B3, B4, B5, C1, C2, D1, D2, E, F1, F2, F3, G, H1, H2, I1, I2, J, K1, K2, K3, K4, K5, L1, L2, L3, M, N, O, P, Q, R, S, T and singletons. Fasta files of whole genome sequences were downloaded from GenBank or the http://phagesdb.org website and TMP nucleotide and amino acid sequences were identified by auto-annotating using DNA Master (http://cobamide2.bio.pitt.edu) when necessary. Gepard [12] was used to generate dotplots of TMP nucleotide and amino acid sequences.