Figure 2

Amplified regions with primer collection and distribution of points of insertion of IS 6110 of M. tuberculosis sequenced genomes (GeneBank). The first circle (blue) represents the distribution of the regions which can be amplified with the primer collection generated in this work. The second concentric circle (fluorescent green) represents the genomic locations of IS6110 in the reference strain H37Rv. The third and fourth circles represent the distribution of this sequence in non-Beijing strains (green) and in Beijing strains (red) respectively and the fifth concentric circle (purple) correspond to the genomic points of insertion of IS6110 in GC1237 used as another reference strain. All the points of insertion and amplified regions are plotted in M. tuberculosis H37Rv genome. The hot-spots: IS1547, plcD region, DR region, dnaA:dnaN region and DK1 region are indicated by arrows. The unique insertion of IS6110 of GC1237 [10] (within Rv2180c) is surrounded by a purple circle.