Table 5 Quantitative PCR primers and conditions
Probe Set ID |   Primer Sequence | Annealing Temp.(C) | Capturing Temp. (C) | Probe Size | Gene Identifier | Identity% |
|---|---|---|---|---|---|---|
Ssc.15995.2.S1_at | GGCATCATGCTGAGTTACATC | 58 | 82 | 106 | KCNE1 | 100 |
AGTAAGCCTTGTCCTTCTCCTG | ||||||
Ssc.10078.1.A1_at | CCTGAGCTGAGAGCCTAGATATG | 58 | 79 | 113 | PCSK5 | 99 |
TTGAATGGCTCCTCTTCAGG | ||||||
 Ssc.1509.1.S1_at | GTTCCAGAACAGCTCGATCC | 58 | 82 | 155 | TNFRSF21 | 98 |
TGCATTGATTCCTTGTGCTC | ||||||
Ssc.17846.1.A1_at | ACGACACAAGCACGTGAGG | 58 | 85 | 157 | IRX1 | 100 |
GGCAACGAAGTCAAGGACTC | ||||||
Ssc.17896.1.A1_at | TCTCCTCCTTCTGCATCAGC | 58 | 81 | 126 | FHOD3 | 100 |
GCACATGGTCCGTTCTTCTC | ||||||
Ssc.17896.2.S1_at | GGCAAGTTCTCTGGCAGTTC | 58 | 84 | 100 | FHOD3 | 98 |
TCAGCACAGCCTTCATGTTC | ||||||
Ssc.19059.1.A1_at | TGCAGTAGCAGGTACAATGGAG | 58 | 78 | 123 | AGTR1 | 99 |
ACCTTGAGAGGAGCAACAGG | ||||||
Ssc.12176.3.S1_at | AGAGTTCTTGCCTTCCTTCG | 58 | 88 | 142 | GPRC5C | 99 |
AGGAGCTTACGACGTCATCC |