Figure 6
mRNA levels of xanthophylls biosynthesis-related genes upon white or blue high light stimulation. The exponentially growing cultures (cell density approximately 5 × 107 cells ml–1) were harvested and transferred cells to 500-ml erlenmeyer flasks (named, 1–9), each containing 250-ml BBM (fresh medium) under continuous white light (390–770 nm) or blue light (420–500 nm) with light intensity of 1,000 μmol photons m–2 s–1 without a day/night cycle, respectively. Collected algal cells (20-mL sample at some selected time) were rinsed with PBS, stored at −80°C if not immediately used. The relative transcript levels of bch [A], Haecyp97a [B], Haecyp97b [C] and Haecyp97c [D] were determined after 2, 4, 6, 10, 13, 24, 28, 34, 48, 54 and 72 h by qRT-PCR using actin as a reference gene. The values were normalized to the transcript levels in the normal light condition. Data are averages of triplicate measurements. The error bars represent standard deviation. Length of the distance in x-axis did not correspond to length of induced time (hours).