Table 1 Comparison of Illumina and modified method
From: Multiplexed Illumina sequencing libraries from picogram quantities of DNA
Parameter | Illumina method | Drawback | Modified method | Benefit |
|---|---|---|---|---|
Adapters | Indexed | Entire sample receives one barcode | Universal | Flexibility; multiple barcodes can be added later |
Size selection | Gel-mediated | Time-consuming; sample loss | No size selection | Time savings; sample retention |
Amplification | 10-18 cycles | Potentially under-optimized | Monitored by qPCR | Stop cycling during log growth phase |
All steps | Constrained by kit | Difficult to modify | Transparent and kit-independent | Flexibility |