Figure 1
From: Genetic basis for phenotypic differences between different Toxoplasma gondii type I strains
Differences in complementation of plaque loss and Irgb6 coating between RH-ERP and GT1 GRA2. (A) Mouse embryonic fibroblasts were stimulated with IFNγ for 24 hours, infected with RH-ERP, RHΔgra2, RHΔgra2+RH-ERP-GRA2, RHΔgra2+GT1-GRA2 and GT1 for 1 hour and stained for Irgb6 (red), Hoechst (blue) and SAG1 (RH-ERP, RHΔgra2 and GT1) or TdTomato (complemented strains) (green). Scale bars represent 10 μm. (B) Quantification of Irgb6 localization on the parasite containing vacuole and percentage plaque loss after 4–7 days on IFNγ stimulated MEFs compared to unstimulated MEFs. Mean + SEM, of at least 4 independent experiments, * p-value < 0.05 and ** p-value < 0.01, Student’s t-test compared to RH-ERP infected MEFs.