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Table 4 Real-time RT-PCR with putative unique transcripts (PUTs)

From: De novo sequencing and transcriptome analysis of the desert shrub, Ammopiptanthus mongolicus, during cold acclimation using Illumina/Solexa

Putative unique transcript ID

Annotation (BLASTX)

Relative gene expression by qRT-PCR (2-ΔΔ CT)

Expression difference analysis of Illumina/Solexa (Log2(CT_RPKM/WT_RPKM))

Unigene3649_All

CBF3 protein [Glycine max]

2.11 ± 0.18

7.8564

Unigene5045_All

PREDICTED: uncharacterized protein LOC100795990 isoform 1 [Glycine max]

2.64 ± 0.51

2.1173

CL9479.Contig1_All

ICE-like protein [Corylus heterophylla]

1.90 ± 0.40

2.2465

Unigene2612_All

PREDICTED: probable transcription factor PosF21-like [Glycine max]

2.70 ± 0.67

4.3792

CL26498.Contig1_All

Basic leucine zipper transcription factor [Medicago truncatula]

4.30 ± 2.79

2.0836

Unigene12211_All

PREDICTED: microtubule-associated protein 70-5-like [Glycine max]

2.82 ± 0.11

2.9144

CL25117.Contig1_All

Cold acclimation protein COR413-PM1 [Medicago truncatula]

3.51 ± 0.32

2.9919

CL33467.Contig1_All

PREDICTED: sugar transporter ERD6-like 5-like [Glycine max]

2.44 ± 0.27

1.9228

Unigene12905_All

PREDICTED: LOW QUALITY PROTEIN: sugar transporter ERD6-like 16-like [Glycine max]

12.59 ± 7.89

10.7104

CL21996.Contig1_All

PREDICTED: cysteine proteinase RD19a-like [Glycine max]

1.78 ± 0.10

1.2868

Unigene28735_All

Medicago truncatula HVA22-like protein a (MTR_4g108350) mRNA, complete cds

2.35 ± 0.28

2.107

Unigene5543_All

HVA22-like protein e [Medicago truncatula]

9.91 ± 2.45

6.7199

Unigene6814_All

PREDICTED: HVA22-like protein k-like [Glycine max]

7.02 ± 0.32

2.8637

Unigene37576_All

PREDICTED: low-temperature-induced 65 kDa protein-like [Glycine max]

2.78 ± 1.34

7.4373

CL11725.Contig1_All

PREDICTED: low-temperature-induced 65 kDa protein-like [Glycine max]

2.16 ± 0.17

7.5431

CL5093.Contig1_All

Hydrophobic protein LTI6B [Medicago truncatula]

2.12 ± 0.10

3.8777

Unigene5480_All

Hydrophobic protein RCI2A [Arabidopsis thaliana]

4.32 ± 0.32

5.8638

CL26053.Contig1_All

PREDICTED: hydrophobic protein LTI6A-like [Glycine max]

3.43 ± 0.26

1.5422

Unigene37577_All

PREDICTED: low-temperature-induced 65 kDa protein-like [Glycine max]

3.53 ± 1.07

6.5686

CL30168.Contig1_All

seed maturation protein [Glycine tomentella]

7.42 ± 0.88

3.3724

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BMC Genomics

ISSN: 1471-2164

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