Figure 5

In vitro validation of selected IL6 correlated genes. Real time RT-PCR on selected IL6-correlated genes was performed using total RNA of starved EOC cell lines untreated (white bars) or treated (grey bars) for 4 hr (IGROV1, OAW42 and IOSE 64 hTERT) or 8 hr (SKOV3) with EGF (20 ng/ml). The number of data sets in which the gene resulted significantly correlated with IL6 is reported on the bottom. Data are mean values (± SD) presented as relative expression normalized for GAPDH mRNA levels. Asterisks indicate significant positive variations (Student’s t test).