Skip to main content
Figure 6 | BMC Genomics

Figure 6

From: Genome-wide identification, molecular cloning, expression profiling and posttranscriptional regulation analysis of the Argonaute gene family in Salvia miltiorrhiza, an emerging model medicinal plant

Figure 6

Experimental validation of miR168a/b- and miR403-mediated cleavage of SmAGO1 and SmAGO2 , respectively. Cleavage sites were determined by the modified 5′ RNA ligase-mediated RACE. Heavy black lines represent ORFs. The lines flanking gray regions represent nontranslated regions. miRNA complementary sites with the nucleotide positions of SmAGO1 and SmAGO2 cDNAs are indicated. The RNA sequence of each complementary site from 5′ to 3′ and the predicted miRNA sequence from 3′ to 5′ are shown in the expanded regions. Watson-Crick pairing is indicated by vertical dashes. Vertical arrows indicate the 5′ termini of miRNA-guided cleavage products, as identified by 5′-RACE, with the frequency of clones shown.

Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us