Figure 9

Reads coverage of the genes in the phn operon. The vertical axis is the number of reads covered at the positions. The orange and dark green bars represent the forward and reverse strands, respectively. Segments with arrows represent genes. Genes from the right to left are yjdN, phnC, phnD, phnE, phnF, phnG, phnH, phnI, phnJ, phnK, phnL, phnM, phnN, phnO and phnP. The graphs were generated using IGB. To make the expression levels for the 14 genes in different samples visible and comparable, the same vertical axis scale (50) is used for the LB and HS treatments, and the same vertical axis scale (450) is used for M-P treatments. Some positions with low read coverage cannot be shown while some other positions with high coverage are truncated. Note the varying levels of coverage and gaps along the operon under different cultures and growth phases, and again the similar position-dependent non-uniform coverage of the reads along the operon.