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Figure 3 | BMC Genomics

Figure 3

From: A comprehensive metatranscriptome analysis pipeline and its validation using human small intestine microbiota datasets

Figure 3

Distribution of mRNA reads assignment. The mRNA reads were assigned to the reference genome database and classified based on their alignment to protein-encoding genes (dark bars) or non-coding (light bars) regions. Based on alignment bit score of mRNA reads to the genome, the reads can obtain phylogenetic and functional identification at genus (blue) and family (green) levels with a minimum bit score of 148 and between 110 and 148, respectively; while the reads with an alignment bit score between 74 and 110 only obtained functional assignments (red). The unassigned reads were represented in black. The specific read numbers that belong to each classification are presented in table S4.

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