Figure 2

Genomic organization of the C. crescentus Fur regulon. The chromosomal clusters of the iron-responsive and Fur-regulated genes are organized in functional categories grouped in separate panels. Genes are also grouped as upregulated (A) or downregulated (B) under both iron limitation and fur mutation. The arrows indicate each open reading frame and their orientation on the chromosome. Differentially expressed genes are indicated in blue. Genes that have been experimentally shown to be directly regulated by Fur [6] are indicated in red. Selected genes that were either iron or Fur regulated (Tables 3, 4 and Additional file 1: Table S1) and showed expression change very close to our cutoff criterion on the other condition are shown in orange. Vertical blocks indicate the location of the Fur binding sites detected by the MEME search described in Figure 1, where sites predicted in silico are white and experimentally validated by EMSA are black [6].