Gut microbiota dysbiosis and bacterial community assembly associated with cholesterol gallstones in large-scale study

Table 1 454 Data summary

	Normal feces	Patient feces	Patient bile	Patient stone	Totally
	(N = 38)	(N = 29)	(N = 26 )	(N = 27)	(N = 120)
Total sequences	77061	64360	81661	76135	299, 217
Sequences per sample	2028 ± 186	2219 ± 175	3141 ± 310	2820 ± 313	2493 ± 127
Total OTUs	1, 497	1, 772	3, 696	3, 456	4, 637
OTU number per sample^***	178 ± 13	167 ± 26	593 ± 73	470 ± 73	321 ± 29
Singletons^***	81 ± 5	78 ± 15	240 ± 44	241 ± 39	166 ± 16
Good coverage (%)	95.1 ± 0.4	96.1 ± 0.5	89.1 ± 1.6	89.7 ± 1.7	92.8 ± 0.6
Shannon diversity index^***	4.97 ± 0.15	4.59 ± 0.21	6.84 ± 0.46	6.25 ± 0.44	5.57 ± 0.18
Chao1^a,***	299.8 ± 20.3	296.9 ± 51.3	1075.9 ± 147.1	847.3 ± 131.1	590.4 ± 54.5
PD - whole tree^a,***	10.4 ± 0.7	11.4 ± 1.4	33.1 ± 3.0	28.3 ± 3.1	19.6 ± 1.4

^*** significant difference of P < 0.001, based on Kruskal-Wallis one way analysis of variance on ranks. ^aPhylogenetic-based alpha diversity measure. PD, Phylogenetic Diversity.
Note: Significant difference was observed between the gut and biliary tract but not within gut or biliary tract, indicating higher microbial diversity in the biliary tract than in the gut.

ISSN: 1471-2164