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Figure 7 | BMC Genomics

Figure 7

From: PuF, an antimetastatic and developmental signaling protein, interacts with the Alzheimer’s amyloid-β precursor protein via a tissue-specific proximal regulatory element (PRE)

Figure 7

EMSA of PRE and selected mutants in NB, PC12, HeLa, NIH3T3, and U373 nuclear extracts. Wildtype oligomers (lanes 1, 5, 9, 13, 17, 21, 25, 29, 33) and oligomers for the M1 (lanes 2, 6, 10, 14, 18, 22, 26, 30, 34), M4 (lanes 3, 7, 11, 15, 19, 23, 27, 31, 35), and M6 (lanes 4, 8, 12, 16, 20, 24, 28, 32, 36) were labeled with γ−32P-ATP, incubated with NB (lanes 1–4) PC12 (lanes 5–8), U373 (lanes 9–12), HeLa (lanes 13–16), HeLa + TPA (lanes 17–20), NIH3T3 (lanes 21–24), NIH3T3 + serum restriction (lanes 25–28), NIH3T3 + IL6 (lanes 29–32), and NIH3T3 + TGFβ (lanes 33–36) nuclear extracts, and analyzed on nondenaturing PAGE, and gels subject to autoradiography.

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BMC Genomics

ISSN: 1471-2164

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