Figure 3

Boxplots for the RT-PCR positive control during analytical and clinical validation studies. Boxplots summarizing performance of each gene over analytical and clinical validation for RT-PCR positive control (prostate cancer FPE pool) representing 18 RT-PCR control plates stratified by gene (ARF1, ATP5E, CLTC, GPS1 and PGK1 are reference genes). Each RT-PCR control plate contains one positive control and each gene is measured in triplicate. Standard deviations ranged from 0.19 Cp to 0.33 Cp. The box represents the inter-quartile range, the line in the box represents the median and the diamond is centered at the mean. The whiskers represent minimum and maximum values observed.