Figure 2

Conservation between Picea mariana unique sequences and peptides from five model plant species and non-redundant protein database: BLASTX searches were performed against: Arabidopsis thaliana, Populus, Vitis vinifera, Oryza sativa , and Physcomitrella patens . (A) Percentage of Picea mariana unique sequences showing similarity with peptides from six databases (> 50 score). Contigs showed the highest number of hits to all five model plant species databases followed by 5' singletons and then 3' singletons. (B-D). 3' singletons, 5' singletons and contigs were analyzed separately using low (score > 50), medium (score > 100) and high (score > 200) BLAST stringency thresholds. (B) The 3' EST singletons had a much greater number of annotations with the model databases in the > 50 score category ranging from 49% to 64%. (C) The 5' EST singletons had the greatest number of hits with scores > 100. (D) The EST contigs had the largest number of high scoring hits (> 50) with representation across most of the five model plant species databases.