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Table 1 Summary of EST sequencing and assembly results

From: Generation, functional annotation and comparative analysis of black spruce (Picea mariana) ESTs: an important conifer genomic resource

EST sequences and contigs

Number

Total EST sequences

4,594

 Number of 5′ sequences

2,455

 Number of 3′ sequences

2,139

Number of contigs

497

Number of singletons

2,234

Average assembled EST length

532.5

Number of full-length cDNA sequences

216

Number of assembled ESTs with:

 

 Significant BLASTX annotations

1,717

 Significant BLASTX annotations with known function

1,478

 No BLASTX annotation information

1,014

Average number of sequences per contig

4.75

Number of contigs containing:

 

 2 ESTs

237

 3 ESTs

91

 4 ESTs

51

 5 ESTs

28

 6 ESTs

17

 7 ESTs

20

 8 ESTs

7

 9-11 ESTs

19

 12-14 ESTs

9

 15-17 ESTs

6

 18-20 ESTs

5

 22-25 ESTs

3

 >25 ESTs

4