Figure 4

Gene dendrogram, module assignment, and correlation to individual traits of interest. The network was created from the weighted correlation matrix generated by WGCNA, by first calculating the adjacency matrix and then calculating topological overlap (TO) to hierarchically cluster genes into coexpression modules (see Materials and Methods). Final module assignments were made based on module membership. (Upper) Cluster dendrogram groups genes into distinct modules. The y-axis represents a dissimilarity distance (1 - TO). Dynamic tree cutting was used to determine modules, by dividing the dendrogram at significant branch points. (Lower) Correlation between individual genes and traits of interest (ethanol consumption and miRNA expression) with FDR < 0.10 are shown as color coded lines: red line indicates positive correlation and blue line indicates negative correlation). Red and turquoise modules (encased by rectangles) appear preferentially targeted by differentially expressed miRNAs.