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Table 2 DNA repair proteins identification by LC-MS/MS

From: DNA repair in Mycoplasma gallisepticum

NCBI ID

Protein name

Unused score*

ProteinPilot score

The number of unique peptides (with the reliability of identification > = 95%)

Coverage protein sequence by unique peptides (with the reliability of identification > = 95%)

gi|31541218

SMC (Cohesin)

84.01

84.01

55

54.43

gi|284811881

UvrA (Excinuclease ABC subunit B)

75.8

75.8

50

55.67

gi|284811888

MGA_0793 (DNA helicase, contain vsr-domain)

62.07

62.07

41

36.41

gi|284812070

UvrD (DNA helicase II)

51.65

51.65

27

47.54

gi|284811857

UvrB (Excinuclease ABC subunit B)

41.75

41.75

24

39.01

gi|31541419

Nfo (endonuclease IV)

31.18

31.18

17

63.04

gi|284812280

LigA (DNA ligase)

24.41

24.83

13

23.08

gi|284812220

RecR (recombinase RecR)

24.06

24.06

15

72.82

gi|284811982

MutM (Formamidopyrimidine-DNA glycosylase)

20.02

20.02

10

41.97

gi|31541551

MGA_0195 (contain endonuclease type II domain)

18.9

18.9

18

49.18

gi|284812101

Hup2 (histone-like protein)

16.7

18.1

23

71.72

gi|284811981

Exo (5′- 3′-exonuclease)

12.01

12.01

8

37.85

gi|284812049

UvrC (Excinuclease ABC subunit C)

6.19

6.19

4

5.672

gi|31541441

MGA_0016 (recombinase RecO)

5.29

5.29

3

19.5

gi|31541171

putative Holliday junction resolvase

4.9

4.9

5

31.69

gi|284812207

DinB (DNA-polymerase IV)

4.05

4.05

2

5.985

gi|31541522

RecA (recombinase RecА)

4.01

4.01

2

8.547

gi|31541659

Ung (Uracil-DNA glycosylase)

3.13

3.86

3

13.85

  1. *The protein identification algorithm receives the value score for the protein as the sum of the scores for all its related peptides (the score in the program is a direct derivative of ProteinPilot reliability of identification). In the case where a peptide is common to the two proteins, its contribution to the score of the protein, which has a lower accuracy of the identification (less than the total score) will be less than the maximum possible value, calculated based on the reliability of his identification. Thus, the value of the unused score reflects the use of the same peptides (or rather the spectrum on the basis of which were identified peptides) in the identification of other proteins. The closer the value of the unused score to the total score of the protein, the more specific and accurate is this identification.