Figure 8

Gene expression changes in BAZ1B -/- cells. (a) Dendrogram (Heat Map) of hierarchical clustering of gene expression between RPE1 and three independent BAZ1B -/- clones. Heat map represents 586 data points, corresponding to probes showing greater than 2-fold change in gene expression at 95% confidence. Expression levels range from +1.4 to +14.2, corresponding to signal intensity on the microarray and represented by a blue (low) to red (high) gradation indicated by the scale bar beneath the heat map. Each column represents the averaged signal from three replicate data sets for each clone. Each row represents a single specific probe. Changes in gene expression can be seen by the difference in row colors between the mutants and parental cells. (b) Representative examples of genes activated in BAZ1B -/- cells by standard end-point RT-PCR analysis shown as an inverted image of an ethidium bromide stained agarose gel. The gene name is given to the left, and the cell line indicated at the top. Lanes show with (+RT) and without (-RT) reverse transcriptase and a water control (H2O).