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Table 3 Validation of microarray data by qRT-PCR

From: Transcriptome analysis of chicken kidney tissues following coronavirus avian infectious bronchitis virus infection

Genes

Accession no

Microarray fold change (infected/control)

Real-time RT-PCR fold change (infected/control)

ISG12-2

NM_001001296

+141.21

+197.36

SPP1

NM_204535

+19.54

+3.66

IRG1

NM_001030821

+21.26

+4.28

STAT1

NM_001012914

+16.57

+14.60

TNFAIP6

NM_001037837

+136.96

+3.33

IFITM3

XM_420925

+422.64

+234.00

SOCS3

NM_204600

+24.75

+9.86

IL6

NM_204628

+97.97

+490.15

IFIT5

XM_421662

+130.57

+84.17

RSAD2

XM_426208

+165.91

+193.13

MX1

NM_204609

+91.194

+16.60

OASL

NM_205041

+185.05

+22.07

  1. qRT-PCR results of 12 immune and inflammatory response related genes in kidney tissues from IBV-infected chickens compared to those of control chickens. qRT-PCR levels of RNA for a given gene were normalized against the 18S RNA. “+” indicates up-regulated genes.