Figure 4

Proposed scheme of for phenylpropanoids and podophyllotoxin biosynthesis in P. hexandrum . A scheme combining annotation by BLASTX, KAAS-KEGG mapping and domain search for phenylpropanoid pathway transcripts and transcripts with domains that may be part of podophyllotoxin biosynthetic pathway from transcripts generated by Newbler default and optimized parameter assembly. PAL-Phenyl alanine ammonia lyase, β-GLUC, Beta glucosidase; C4H, cinnamate 4-hydroxylase; HCT, Hydroxy cinnamoyl transferase; 4CL, 4-coumarate ligase; CCR, cinnamoyl CoA reductase; CAD, Cinnamoyl alcohol dehydrogenase; POD, Peroxidase; SAD, Sinapyl alcohol dehydrogenase; SDR, Secoisolariciresinol dehydrogenase like; PCBER, phenylcoumaran benzylic ether reductase like; AdoMeT, MTase-Adenosyl Methionine dependent methyl transferase like; ISOPREN-C2-like, Isoprene-C2-like reductase; URO-D-CIMS-like, Uroporphyrinogen decarboxylase methyl transferase.