Figure 2
Morphology, morphology scores, and heart rate – morphology correlations of reference and resistant embryos. A) Embryo Morphology: progression of deformities among exposed reference embryos: 1 = control embryo; 2 = 1 μg/L BNF –exposed embryo; 3 = 50 μg/L ANF – exposed embryo; 4 = 100 μg/L ANF – exposed embryo; 5 = 1 μg /L BNF + 50 μg/L ANF – exposed embryo; 6 = 1 μg/L BNF + 100 μg/L ANF – exposed embryo. A – atrium; BI – blood island; E – eye; CD – cranial deformity (reduced cranial width and eye distance, diminished cranial ridges); HM – hemorrhage; M – melanin; PE – pericardial edema; TH – tube heart; V – ventricle; B) Embryo deformity assessment among reference (blue) and resistant (red) embryos under 6 treatments. 2-way ANOVA (p < 0.010 and Bonferroni post-test (p < 0.01) revealed statistical differences in 4/6 treatments between embryo populations. Asterisks (*) represent statistically significant within-treatment differences (Bonferroni post-test, p < 0.05) between Elizabeth River (ER - resistant) and King’s Creek (KC - reference) embryos. C) Correlation between Embryo Morphology and Heart Rate: A) Strong correlation (R2 = 0.82) is apparent among reference embryos; B) No correlation is apparent among resistant embryos (R2 = 0.044); C) Combined data of reference and resistant embryos shows strong correlation between progression of deformities and decrease in heart rates (R2 = 0.78).