Figure 1

The effect of NtTTG2 silencing on tobacco growth. The NtTTG2-silenced tobacco line TTG2RNAi4 was compared to the control WT RFP plant in terms of the vegetative growth and the expression of NtTTG2 and EXP genes. (a, b) Root monitoring after 10-day growth on the medium. (c) Plants grown in pots. Floral development stages S1–S5 are indicated. (d, e) The biomass of plants from (c). (f) Real-time RT-PCR analyses of the indicated genes expressed in roots of plants from (b). (g) Real-time RT-PCR analyses of gene expression in leaves of 45-day-old plants grown in pots and equivalent to those in (c). In (b, d-g), data shown are mean values ± standard deviation (SD) bas of results from five experimental repeats (50 plants/repeat). Data were analyzed by the ANOVA method along with one-way Fisher’s Least Significant Difference (LSD) test. Significant differences between data pairs at the corresponding time points are indicated by single asterisks (P < 0.05) or double asterisks (P < 0.01) on the graphs.