Figure 1

Phenotypic evaluation of different haplotypes carrying or not recombination events within/next to the confidence interval of Mt-FTQTL6. F₇ and F₈ seedlings were obtained from the self-pollination of the homozygous F₆ and F₇ lines whose code names are indicated in the ‘Mother plant identifier’ column. These lines originally derive from a cross between M. truncatula accessions F83005-5 [freezing-tolerant] and DZA045-5 [freezing-sensitive] (see ‘Development of plant material’ in Methods). The genotypes of F₇/F₈ lines at Mt-FTQTL6 are provided based on information from 26 markers that are shown according to their established genetic and/or physical order ([39]; this study). Green bars refer to chromosomal regions harboring alleles from F83005-5. Orange dotted bars represent regions with alleles from DZA045-5. Markers bordering the confidence interval of Mt-FTQTL6 according to [39] are underlined. These are 5.7 cM distant [39]. Haplotype groups A-I contain the recombinant and non-recombinant F₇/F₈ lines that were compared between each other. The mean freezing damage score (FDS) of each line is provided in the ‘Freezing tolerance assessment’ part of this figure. Pairwise comparisons of mean FDS were conducted between recombinant lines, respective controls (if any), F83005-5 and DZA045-5 (see ‘Evaluation of freezing tolerance’ in Methods). Letters (a) and (b) are used to distinguish significantly different FDS in each haplotype group. Due to the lack of individuals representing the parental accessions, the mean FDS of the progeny of recombinant line 76-11-108-04 in group I was only compared with those of the progenies of 76-11-108-07 and 76-11-51-08, considered as positive and negative controls respectively. Deduced phenotypes of F₇/F₈ lines are indicated to the right of the figure: ‘S’, sensitive to freezing; ‘T’, tolerant to freezing. Grey-highlighted markers in the ‘Genotype at Mt-FTQTL6’ part are those contained in Mt-FTQTL6’s confidence interval delimited according to genotype and phenotype data from screened lines.