Figure 4

Polymorphism between F83005-5 and DZA045-5 in the coding sequences of MtCBF2 - 3 ; 5 - 12 ; 14 . The order of sequences reflects that of the respective genes in Mt-FTQTL6 region. Schematic representations of the coding sequences of MtCBF2-3;5-12;14 are based on the shortest allele (F83005-5 or DZA045-5) at each time. Blue boxes illustrate regions encoding AP2/ERF domains while orange boxes depict coding regions for characteristic CBF/DREB1 signature sequences, i.e. (from left to right) PKKP/RAGRxKFxETRHP, DSAWR, A(A/V)xxA(A/V)xxF and LWSY. Both alternatively spliced isoforms of MtCBF6 and MtCBF8 are shown. MtCBF2a and MtCBF2b, obtained from F83005-5 using primer sets designed on the same gene sequence from A17, are both compared to the unique sequence obtained from DZA045-5 with these primers. Black and red horizontal lines indicate the positions of synonymous and non-synonymous SNP, respectively. Dotted horizontal lines indicate the positions of indel or SSR polymorphisms. Arrows are used to delimit comparable sequences between F83005-5 and DZA045-5 in case only a partial sequence could be obtained from one or both accessions. Asterisks followed by letters “F” or “D” show the positions of stop codons for F83005-5 and DZA045-5, respectively.