Table 3 PCR assays, including primers sequences, accession numbers, amplicon sizes and PCR efficiencies
Gene | Gene product | Accession no. | Forward primer | Reverse primer | Amplicon size (bp) | PCR efficiency* |
|---|---|---|---|---|---|---|
CuZn SOD | CuZn superoxide dismutase | BG936553 | CCACGTCCATGCCTTTGG | TCAGCTGCTGCAGTCACGTT | 140 | 1.92/2.02 |
Mn SOD | Mn superoxide dismutase | DY718412 | GTTTCTCTCCAGCCTGCTCTAAG | CCGCTCTCCTTGTCGAAGC | 209 | 1.85/1.88 |
CAT | Catalase | BG935638 | GGGCAACTGGGACCTTACTG | GCATGGCGTCCCTGATAAA | 59 | 1.85/2.12 |
GPX1 | Glutathione peroxidase 1 | EH033571 | TCTCCTGCCATAACGCTTGA | GTGATGAGCCCATGGCCTTA | 137 | 1.84/- |
GR | Glutathione reductase | BG934480 | CCAGTGATGGCTTTTTTGAACTT | CCGGCCCCCACTATGAC | 61 | 2.00/1.91 |
HSP70 | Heat shock protein 70 | BG933934 | CCCCTGTCCCTGGGTATTG | CACCAGGCTGGTTGTCTGAGT | 121 | -/1.90 |
HIF1A | Hypoxia-inducible factor 1A | DY708816 | CCACCTCATGAAGACCCATCA | TCTCCACCCACACAAAGCCT | 101 | 2.20/2.26 |
IGFBP1A | Insulin-like growth factor binding protein 1A | KC122927 | GGTCCCTGTCATGTGGAGTT | TTCCAGAAGGACACACACCA | 184 | 2.10/2.08 |
IGFBP1B | Insulin-like growth factor binding protein 1B | AY662657 | GAGGACCAGGGACAAGAGAAAGT | GCACCCTCATTTTTGGTGTCA | 101 | -/2.02 |
MTOR | Mechanistic target of rapamycin (serine/threonine kinase) | BT072258 | CAGCCTGAGGCCCTGAATAA | CTCCACTTGGGTTGGCACAT | 114 | 1.97/1.95 |
CYP1A | Cytochrome P450, family 1, subfamily A | >contig00118 length = 2495 numreads = 57 | ATC GGACGCAACGAGGTCTA | TGACAGCGCTTGTGCTTCAT | 128 | 1.97/2.02 |
NDUFS1 | NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75 kDa | >contig00384 length = 2136 numreads = 57 | TGCTGCAGGACATCGCTAAC | TGGTTTGCACAGAGCTCAAGA | 135 | 1.94/2.01 |
PSMC2 | Proteasome (prosome, macropain) 26S subunit, ATPase, 2 | >contig01910 length = 1544 numreads = 106 | ATCAGGGTCATCGGCTCAGA | GCCCCTCCAATAGCGTCAAT | 132 | 1.94/2.02 |
HSP90B | Heat shock protein 90B | >contig03769 length = 1183 numreads = 111 | CCACCATGGGCTACATGATG | CCTTCACCGCCTTGTCATTC | 114 | 1.97/1.95 |
EEF1AB | Eukaryotic translation elongation factor 1AB (refgen) | AF321836 | CCCCTCCAGGACGTTTACAAA | CACACGGCCCACAGGTACA | 57 | 1.99/2.01 |
ACTB | Beta-actin (refgen) | BG933897 | CCAAAGCCAACAGGGAGAA | AGGGACAACACTGCCTGGAT | 102 | 2.06/1.90 |
RPL13 | Ribosomal protein L13 (refgen) | NM_001141291 | CCAATGTACAGCGCCTGAAA | CGTGGCCATCTTGAGTTCCT | 110 | -/1.91 |