Figure 2

Example of a gene fusion identified in the Ivy Center SOLiD single-end data. This figure illustrates the MON2-MARS gene fusion. A. Outlier expression of the MON2 and MARS exons can be observed with a z-score > 4. MON2 read distribution shows a drop after exon 34, and MARS read distribution shows a rise at exon 6. Partially mapped reads at exon 34 of MON2 and exon 6 of MARS map to the MON2-MARS fusion sequence. The sequence is represented both in color space and base space format. The purple color indicates a mismatch in the color space format. B. Gel images showing the fusion qPCR result. The product can be observed in sample SN214 (TCGA-74-6583) but not in the non-tumor brain and MON2-MARS fusion negative GBM samples. C. Trace from Sanger sequencing of the fusion PCR product.