Figure 4

Characteristics of the vR26 and the chimeric vR26_E2gif in cells. (A) Virus yield assays were performed during passage (from passage 1 to 12) of vR26 and vR26_E2gif in PK15 and SFT-R cells as indicated. For each passage, cells were harvested after 3 days and the virus titres determined. Data are presented as mean + SD (n = 2). (B) Cells (SFT-R) infected with the vR26/P-12 or with the vR26_E2gif /P-12 viruses were stained using a polyclonal anti-pestivirus serum (which recognizes both BDV and CSFV proteins) and with specific mAbs directed against the CSFV E2 (WH211) and BDV E2 (WB166) proteins as indicated.