Figure 5

Phylogenetic analyses of salivary Psorophora protein families previously found exclusively in Aedes/Ochlerotatus mosquitoes. Clustal alignment of all Aedes 6.5–8.5-kDa proteins (A) and Aedes W-rich peptides (B) derived from Psorophora sialotranscriptome. Symbols above the alignment indicate (*) identical sites, (:) conserved sites, and (.) less-conserved sites. Aedes 34-kDa family alignment and bootstrapped phylogram (C). The numbers on the tree bifurcations indicate the percentage bootstrap support above 75%. The scale bar at the bottom represents 10% amino acid substitution. Sequences were aligned by the ClustalW program, and the dendrogram was made with the Mega package after 10,000 bootstraps with the neighbor-joining algorithm.