Table 2 Overall mismatch rates in overlapping paired-end reads for raw read pairs, read pairs that have quality scores Q10, Q≥20, Q≥30 and Q≥35
From: Ultra-deep mutant spectrum profiling: improving sequencing accuracy using overlapping read pairs
Sample | Raw | Q10 (1×10-1) | Q20 (1×10-2) | Q30 (1×10-3) | Q35 (3×10-4) |
|---|---|---|---|---|---|
BCV control | 5.55×10-3 | 7.82×10-4 | 9.56×10-5 | 1.94×10-6 | 4.26×10-8 |
Rabies control | 7.33×10-3 | 9.64×10-4 | 1.12×10-4 | 2.59×10-6 | 1.31×10-7 |
BCV | 4.91×10-3 | 7.87×10-4 | 1.06×10-4 | 2.55×10-6 | 1.51×10-7 |
Fox1 | 8.92×10-3 | 1.01×10-3 | 1.73×10-4 | 8.82×10-6 | 6.92×10-7 |
Fox2 | 6.69×10-3 | 8.33×10-4 | 1.22×10-4 | 4.16×10-6 | 2.28×10-7 |
Average of 5 samples | 6.68×10-3 | 8.75×10-4 | 1.22×10-4 | 4.01×10-6 | 2.49×10-7 |
Rabies control –repeat run | 5.99×10-3 | 2.90×10-3 | 1.04×10-3 | 2.67×10-4 | 8.00×10-5 |