From: Ultra-deep mutant spectrum profiling: improving sequencing accuracy using overlapping read pairs
Natural sample | Mean ORP coverage | # candidate variant calls at Q≥30 | # variants called at error rate 5×10-5 | # variants called at error rate 5×10-4 | FDR for error rate 5×10-5 | FDR for error rate 5×10-4 |
---|---|---|---|---|---|---|
BCV | 79,037x | 20413 | 2133 | 152 | 19% | 0% |
Fox1 | 80,546x | 14472 | 1354 | 70 | 21% | 0% |
Fox2 | 96,198x | 20417 | 1596 | 88 | 26% | 0% |