Volume 14 Supplement 6
A novel in silicoreverse-transcriptomics-based identification and blood-based validation of a panel of sub-type specific biomarkers in lung cancer
- Debmalya Barh1Email author,
- Neha Jain1, 2,
- Sandeep Tiwari1,
- John K Field3,
- Elena Padin-Iruegas4,
- Alvaro Ruibal5,
- Rafael López4,
- Michel Herranz6,
- Antaripa Bhattacharya1,
- Lucky Juneja1, 2,
- Cedric Viero1, 7,
- Artur Silva8,
- Anderson Miyoshi9,
- Anil Kumar2,
- Kenneth Blum1, 10,
- Vasco Azevedo9,
- Preetam Ghosh1, 11 and
- Triantafillos Liloglou3
© Barh et al.; licensee BioMed Central Ltd. 2013
Published: 25 October 2013
Lung cancer accounts for the highest number of cancer-related deaths worldwide. Early diagnosis significantly increases the disease-free survival rate and a large amount of effort has been expended in screening trials and the development of early molecular diagnostics. However, a gold standard diagnostic strategy is not yet available. Here, based on miRNA expression profile in lung cancer and using a novel in silico reverse-transcriptomics approach, followed by analysis of the interactome; we have identified potential transcription factor (TF) markers that would facilitate diagnosis of subtype specific lung cancer. A subset of seven TF markers has been used in a microarray screen and was then validated by blood-based qPCR using stage-II and IV non-small cell lung carcinomas (NSCLC). Our results suggest that overexpression of HMGA1, E2F6, IRF1, and TFDP1 and downregulation or no expression of SUV39H1, RBL1, and HNRPD in blood is suitable for diagnosis of lung adenocarcinoma and squamous cell carcinoma sub-types of NSCLC. Here, E2F6 was, for the first time, found to be upregulated in NSCLC blood samples. The miRNA-TF-miRNA interaction based molecular mechanisms of these seven markers in NSCLC revealed that HMGA1 and TFDP1 play vital roles in lung cancer tumorigenesis. The strategy developed in this work is applicable to any other cancer or disease and can assist in the identification of potential biomarkers.
Lung cancer is the leading cause among cancer related deaths worldwide, constituting 17% of new cancer cases and 23% of deaths from cancer. Although N. American and European countries show a slow decline in death rates due to lung cancer, deaths due to this form of cancer are increasing considerably in Asian and African countries . Lung cancer is mainly divided into two subtypes, small cell lung cancer (SCLC), which accounts for 10-15% of all cases and non-small cell lung cancer (NSCLC, 85-90%). The latter group is further histologically subdivided into four categories; adenocarcinoma, squamous cell carcinoma, large cell carcinoma and 'others', for example cancers of neuroendocrine origin . The overall 5-year survival rate for NSCLC ranges from 9% to 15% . The high mortality from lung cancer is due a combination of lack of reliable early diagnostic tools [3, 4] along with a poor arsenal of lung cancer regimens for stage I lung cancer, whose survival rate is also surprisingly low .
Numerous studies have utilized different "-omics"-based approaches to identify molecular signatures in lung cancer with diagnostic or prognostic value while using minimally invasive processes. Some of these are as follows: 34 miRNA signatures , expression profiles of 11 miRNAs (miR-106a, miR-15b, miR-27b, miR-142-3p, miR-26b, miR-182, miR-126, let7g, let-7i and miR-30e-5p) from serum , 7 miRNA signatures , overexpression of six snoRNAs , and expression of 3 miRs (miR-205, miR-210 and miR-708) in sputum . Additional signatures and markers have also been reported from the plasma proteome [11, 12], the salivary proteome , the serum epigenome , sputum-based genomics , and blood-based gene expression studies . However, none of these have progressed sufficiently to provide the necessary specificity and sensitivity required for clinical implementation.
microRNAs (miRNAs/miRs) are involved in a variety of biological processes, including cell cycle regulation, cell differentiation, development, metabolism, and aging . They have also been shown to be aberrantly expressed in several cancers . Lung cancer is no exception to this and miRNA signatures have been suggested to be useful in diagnosis, prognosis, and therapy [7, 19–21]. miRNAs regulate posttranscriptional gene expression and a single miRNA can regulate up to 200 mRNAs including those for transcription factors (TFs) . Because miRNA transcription is under the regulation of TFs, intriguing feed-back and feed-forward regulatory loops can be formed among TFs and miRNAs .
In this study we have developed a novel in silico reverse-transcriptomics strategy followed by interactome analysis to identify the sub-type specific diagnostic TF markers in lung cancer. The approach is novel as the sub-type specific TF markers were identified starting with experimentally validated miRNA profiles in lung cancer. We have also attempted to provide a molecular insight during the early events in lung cancer.
Materials and methods
Extensive literature and text mining was carried out to collect deregulated miRNAs in lung cancers (NSCLC and SCLC) using databases such as PubMed, Sirus, and Elsevier as well as search engines such as Google and Google Scholar. miR2Disease  was also used to gather lung cancer specific miRNAs information. Priority was given to reports that have used markers based on biopsy samples and patient's remote media (blood, serum, plasma, sputum, and bronchioalveolar lavage among others ). Selected miRNAs were then grouped into three categories: (1) NSCLC specific, (2) exclusively SCLC related, and (3) common in both the types. The up- and down-regulated miRNAs within each of these three groups were also noted.
GO assignment to miRNAs using reverse annotation strategy
miRNA-TF-miRNA or TF-miRNA-TF interactions
To date, there is no study reporting direct miRNA-miRNA interaction. However, it is well known that miRNAs can modulate post-transcriptional gene regulation as well as their own expression through feed-back and feed-forward loops that are mediated by various TFs. Therefore, there are miRNA-TF interactions. As TFs interact with other TFs and proteins, the known TF-TF networks can be complemented by integrating the relevant miRNA-TF interactions to make TF-miRNA-TF or TF-miRNA-TF-miRNA interactions. Such TF-miRNA-TF-miRNA interaction networks will indirectly represent the miRNA-miRNA interactions.
We thus created a cancer specific TF-TF interaction network using targets of miRNAs frequently deregulated in NSCLC, SCLC, or common to both of these types utilizing Osprey v1.0.1  (Figure 1, Step-3). To achieve this, we selected all experimentally validated, highly ranked miRNA targets of NSCLC, SCLC, or common to both that were identified in the previous step and fed them into Osprey (Figure 1, Step-6). The protein-protein interaction (PPI) network for each cancer type generated by Osprey was first filtered sequentially with the "Transcription", "Cell cycle" and "Cell cycle biogenesis" GO filters in Osprey (Figure 1, Step-8). Therefore, the resultant TF-TF interaction network is cell cycle specific. The sequential filters were used because cell cycle deregulation is one of the major BPs (Biological Processes) that is affected during tumorigenesis.
This cell cycle specific TF-TF network was further enriched by manually mapping the interacting miRNAs with data collected from the miReg , TransmiR , and CircuitsDB  databases and from literature mining to create a TF-miRNA-TF interaction map (Figure 1, Step-10). Because we have selected lung cancer related miRNAs (based on GO assignment in the previous step) and developed a network using their targets, this network represents the interaction of TFs involved in lung cancer tumorigenesis. Based on our earlier hypothesis, this interaction map also represents the miRNA-TF-miRNA or TF-miRNA-TF interaction map that is common to both NSCLC and SCLC. Similarly, NSCLC and SCLC specific miRNA-TF-miRNA or TF-miRNA-TF or miRNA-miRNA interaction maps were created using targets of NSCLC and SCLC unique miRNAs. Therefore, a total of three networks were generated (Figure 1, Steps-14-15).
The miRNA-TF-miRNA or TF-miRNA-TF interaction maps for NSCLC, SCLC, and common developed in the previous steps were analyzed by subtracting from each other to identify the NSCLC, SCLC, and a common pathway that is specific unique TFs. Each network was further analyzed using the protein-protein interaction (PPI) analysis tool VisANT  to identify the key nodes and the shortest cancer specific pathways in each network. Key nodes in a PPI network are identified as having the highest number of interactions. Therefore, such key node proteins are often involved in multiple signaling pathways, and if a key node protein falls in a shortest path, the node might be treated as a marker of a disease provided that its expression is altered in that disease state. In the third strategy, we utilized GSEA identification of key genes in each network using ToppGene Suite . When all of the data from each of these three analyses had been obtained, we identified the TFs common to each of the individual analyses (Figure 1, Steps-11-12). Therefore, these sets of common TFs were putative markers, and the TFs that were a part of NSCLC network could be treated as a NSCLC-specific marker.
Experimental validation of markers
Once we had selected the potential markers, we checked their expression levels initially in lung cancer tissue samples using microarrays and then further validated them using patient's blood samples and quantitative RT-PCR (qPCR) (Figure 1, Step-13).
Interrogation of data from expression microarray
The frozen tissue samples examined from 30 squamous cell carcinomas and 30 adenocarcinomas (each is a type of NSCLC) from the Liverpool Lung Project tissue bank. All samples were of pathological stage T2. RNA was extracted using the RNeasy kit (Qiagen). Five RNA pools from five adjacent normal lung tissues were also profiled for comparison purposes. The microarray experiments were performed by Almac (Belfast, UK). Total RNA was amplified using the NuGEN™ Ovation™ RNA Amplification System V2. First-strand synthesis of cDNA was performed using a unique first-strand DNA/RNA chimeric primer mix, resulting in cDNA/mRNA hybrid molecules. Following fragmentation of the mRNA component of the cDNA/mRNA molecules, second-strand synthesis was performed, and double-stranded cDNA was produced with a unique DNA/RNA heteroduplex at one end. In the final amplification step, RNA within the heteroduplex was degraded using RNaseH, and a replication of the resultant single-stranded cDNA was achieved using the DNA/RNA chimeric primer binding and DNA polymerase enzymatic activity. The amplified single-stranded cDNA was purified to allow accurate quantitation of the cDNA and to ensure optimal performance during the fragmentation and labeling process. The single-stranded cDNA was assessed using spectrophotometric methods in combination with the Agilent Bioanalyzer.
The appropriate amount of amplified single-stranded cDNA was fragmented and labeled using the FL-Ovation™ cDNA Biotin Module V2. The enzymatically and chemically fragmented product (50-100 nt) was labeled via the attachment of biotinylated nucleotides onto the 3'-end of the fragmented cDNA.
The resultant fragmented and labeled cDNA was added to the hybridization cocktail in accordance with the NuGEN™ guidelines for hybridization onto Affymetrix GeneChip® arrays. Following hybridization for 16-18 hours at 45°C in an Affymetrix GeneChip® Hybridization Oven 640, the array was washed and stained on the GeneChip® Fluidics Station 450 using the appropriate fluidics script and then inserted into the Affymetrix autoloader carousel and scanned using the GeneChip® Scanner 3000.
The Rosetta Error Model has been applied to the raw data to generate the processed data. The profile comparisons between cancerous lesions and normal RNA pools utilized Student's t-test. The Benjamini & Hochberg multiple test correction method was also employed.
Validation using quantitative RT-PCR (qPCR)
Blood samples, RNA isolation, and cDNA preparation
As our focus is NSCLC, blood samples from 8 metastatic lung adenocarcinoma, 8 metastatic squamous cell lung carcinoma patients, and 5 healthy volunteers (control) were used for the validation. Patient eligibility criteria were as follows: 18 years of age or older, in clinical stage II-IV based on the International TNM classification, performance status of 0 to 2, and no other malignances. All patients and volunteers have signed informed consent forms. Ten milliliters of EDTA blood sample was collected from the selected groups before chemotherapy treatment. Blood samples were centrifuged at 2000 g for 10 min and the serum phase was separated and frozen at -80ºC. The Buffy Coat (white blood cells and circulating tumor cells) was collected and processed by lysis (Ammonium Chloride, TRIS, ddH20) and then washed with PBS. The dry pellet was kept at -80ºC until RNA isolation. RNA was purified by Quiamp RNA Blood Mini Kit (QIAGEN Inc., USA) according to the manufacturer´s instructions. cDNA was synthesized with random hexamer primers (Deoxynucleoside Triphosphate set, Roche, Germany) at 10 mM, MgCl2, MuLV Reverse Transcriptase, PCR Buffer, RNAse Inhibitor, and random hexamers from Applied Biosystems USA. The resulting cDNA was stored at -20ºC until further use.
Quantitative RT-PCR (qPCR)
qPCR was carried out using SYBR® Green Master Mix (Applied Byosistems, USA) and Applied Biosystem's 7500 real-time PCR system according to the manufacturer´s instructions. Primers for GAPDH were designed with Vector NTI Advance™ 11 (Invitrogen) and primers for TFDP1, SUV39H1, RBL1, E2FG, IRF1, HMGA1, and HNRPD were designed using qPrimerDepot (http://primerdepot.nci.nih.gov/). To avoid the influence of genomic contamination, the amplicons spanned at least one intron. The primers used are listed in Additional file 1. qPCR was performed in a final volume of 20 µl with a SYBR PCR Master Mix, using 1 µl cDNA. Cycling conditions were 95ºC for 10 min, followed by 40 cycles at 95ºC for 15 s and 60ºC for 1 min each to obtain the melting curve.
Relative gene expression levels were determined by the quantitative curve method. Quantitative normalization of the cDNA in each sample was performed using GAPDH gene expression as an internal control. Target gene mRNA levels were given as ratios to GAPDH mRNA levels. qPCR assays were performed in duplicate for each sample, and the mean value was used to calculate the mRNA expression levels.
miRNA statistics in lung cancer
We selected 184 miRNAs for NSCLC and 62 for SCLC using literature mining and the miR2 Disease database. Among these 246 miRNAs, 41 were found to be involved in both of the lung cancers and therefore are common miRNAs involved in lung cancer regardless of the subtype (Figure 1, Step-1). In the common miRNA group, 13 and 11 miRNAs were found to be up- and downregulated, respectively; whereas 18 miRNAs showed differential expression, i.e., either upregulated in SCLC and downregulated in NSCLC or vice versa (Figure 1, Step-2) (Additional file 2). A total of 22 miRNAs were found to be unique to SCLC (16 upregulated and 6 downregulated) (Additional file 3). For NSCLC, the total number of unique miRNAs was 143, (89 upregulated and 43 downregulated) (Additional file 4).
Target-based functional annotation of miRNAs
Using miRWalK, miRBASE, miRecord, miRTarBASE, and miReg we identified several validated targets for each miRNA. Thereafter, as per our reverse transcriptomics strategy, targets for each miRNA were subjected to gene enrichment analysis using ToppGene Suite as described in Materials and Methods (Figure 1, Step-3). Top targets that are associated with common, NSCLC, and SCLC were identified. DAVID-based functional annotations of the top targets revealed that most of these targets are cell cycle related, so the miRNAs that have these targets are related to transcription, cell cycle regulation, cell biogenesis and organization, cell proliferation, and other biological processes related to tumorigenesis. The list of common miRNAs involved in lung cancer along with their corresponding GO terms is presented in Additional file 5. miRNAs involved uniquely in either NSCLC or SCLC and their corresponding GO terms were also defined (data not shown).
miRNA-miRNA interaction network in lung cancer
Interaction of common miRNAs
Interactions of SCLC associated miRNAs
Interactions of NSCLC linked miRNAs
SCLC network is a part of NSCLC
Next we subtracted the LC specific networks from each other to identify unique network specific TFs (Figure 1, Step-11). In the 27 nodes of the NSCLC network (Figure 4), all of the SCLC nodes (Figure 2) are found to be present (Figure 4, in red circle). Therefore, it is evident that there are additional pathways involved in NSCLC compared to SCLC and the SCLC network represents a subset of the NSCLC network.
Genes involved in common events in lung cancer
Identified putative markers in lung cancers using the in silico reverse transcriptomics approach.
Unique to SCLC
RB1, E2F1, E2F2, CCNT2, CMYC, CEBPA, TP53, CDKN2A, HDAC4
Common to SCLC and NSCLC
RB1, E2F1, E2F2, CCNT2, CMYC, CEBPA, TP53, CDKN2A, HDAC4
Common to general, SCLC, and NSCLC
RB1, E2F1, E2F2, CCNT2, CMYC, CEBPA, TP53, CDKN2A, HDAC4
Common to NSCLC and general
TFDP2, AHR, CCND1, TP73, RBL2, TAF1, PML, BCL6, MYB, WT1, PARP1, PCAF, TWIST, MCM7
E2F6, TFDP1, SUV39H1, HNRPD
General/ common path specific
RBL1, IRF1, HMGA1
Validation of markers
We selected seven genes [4 unique genes (E2F6, TFDP1, SUV39H1, and HNRPD) for NSCLC and 3 genes (RBL1, IRF1, and HMGA1) for general events] for validation as diagnostic markers in lung cancer. Frozen NSCLC tissue-based microarray analysis revealed that E2F6, TFDP1, SUV39H1, and HMGA1 are significantly upregulated in both the adenocarcinoma and squamous cell carcinoma samples. The upregulation of RBL1 and downregulation of IRF1 in the microarray analysis was significant in squamous cell carcinoma but was statistically insignificant in adenocarcinoma (Additional file 6).
In this work we have identified key transcription factors that can be useful biomarkers in diagnosis of lung cancer using an in silico reverse-transcriptomics approach. In this novel approach, starting with deregulated miRNAs in lung cancers we have identified transcription factors that can act as biomarkers, even for sub-type specific lung cancers. Out of several putative markers we identified, 7 NSCLC specific markers were validated. We found that E2F6, HMGA1, IRF1, and TFDP1 were upregulated and RBL1, SUV39H1, and HNRPD were downregulated or aberrantly expressed in adenocarcinoma and squamous cell carcinoma, which are the sub-types of NSCLC.
HMGA1 (High mobility group AT-hook 1) is an oncogene that is induced by Wnt/beta-catenin pathway and which positively regulates cell proliferation in gastric cancer . By downregulating E-cadherin and upregulating expression of TWIST1, it enhances epithelial-mesenchymal transition and metastasis in colon cancer . Upregulation of HMGA1 in glioblastoma positively correlates with malignancy, angiogenesis, and invasion . In lung cancer, it is also overexpressed and increased nuclear expression correlates with poor survival in lung adenocarcinomas [37, 38]. By upregulating PI3K and MMP2, it promotes cell migration and invasion [37, 39] and by activating miR-222 oncomiR, it induces PPP2R2A mediated AKT signaling in NSCLC . Therefore, upregulation of HMGA1 plays a significant role in tumor progression in NSCLC. In our study, we also observed that HMGA1 was upregulated in NSCLC supporting the previous findings.
TFDP1 (Transcription factor Dp-1) is a candidate oncogene that positively regulates S-phase entry and inhibits apoptosis in cooperation with E2F1 . It is amplified and overexpressed in breast cancer  and upregulation of TFDP1 positively correlates with tumor size and progression of hepatocellular carcinomas  and increased cell viability in lung cancer . In our observation, TFDP1 was overexpressed in all lung adenocarcinomas and squamous cell carcinomas, which supports the previous findings of Lu et al. (2000) in a SCLC cell line .
In our study, we observed IRF1 (Interferon regulatory factor 1) was upregulated in all NSCLC samples tested, although it had been shown to be downregulated in lung cancer in a previous study . IRF1 inhibits G1-S cell cycle progression through P53 and p21 mediated pathways  and may act as a tumor-suppressor gene. This finding is supported by the findings that it is downregulated in gastric  and recurrent breast cancers . However, IRF1 may not always act as a tumor-suppressor, as there is a report that it is upregulated in skin squamous cell carcinoma . Therefore, our observation of upregulated IRF1 in NSCLC samples requires further attention to explore the precise role of this TF in various cancers.
E2F6 (E2F transcription factor 6) inhibits entry into S phase of cells stimulated to exit G0  and inhibits apoptosis through E2F1 . It may therefore play a role in cell proliferation and cell survival. There is no report about this protein's expression pattern in any cancer. Here, we have, for the first time, observed that E2F6 was upregulated in all of our tested NSCLC samples. This finding supports E2F6's putative role in tumorigenesis and shows that it may be a novel marker for NSCLC.
SUV39H1 (Suppressor of variegation 3-9 homolog 1) is a histone methyltransferase that inhibits inflammatory responses by downregulating interleukin-6 production . SUV39H1 inhibits the expression of CCND1 and may thereby negatively regulate cell proliferation . However, its overexpression induces cell migration in breast and colon cancers  and negatively regulates apoptosis in a lung cancer model . The expression level of SUV39H1 inversely correlates with stage, prognosis, and disease free survival in oral squamous cell carcinoma  and breast cancer . Therefore, SUV39H1 may also have oncogenic properties. Although SUV39H1 was significantly upregulated in adenocarcinoma and squamous cell carcinoma tissue samples in our microarray analysis, supporting its positive role in tumorigenesis, it was found to be downregulated in blood samples in our qPCR validation. Therefore, SUV39H1 expression differs in lung cancer tissue and blood samples.
RBL1 (Retinoblastoma-like 1 (p107)) inhibits cell proliferation through G1 arrest  and positively regulates epidermal differentiation . RBL1 is downregulated and inversely correlates with the histological grade of squamous cell carcinomas and adenocarcinomas . Our qPCR validation shows downregulation in all squamous cell carcinoma and adenocarcinoma samples, which supports the previous findings and RBL1's function in tumors.
HNRPD/AUF1 is a RNA-binding protein that both positively and negatively regulates neoplastic gene regulatory networks in cancer depending on the type of neoplasm . It binds to destabilize p21 mRNA and thereby inhibits its anti-apoptotic activity . Although in our blood-based qPCR analysis AUF1 was downregulated in all NSCLC samples, it has been reported to be upregulated in HCC  and experimental murine lung cancer . It has been patented to aid in the prediction of survival in lung cancer in a gene expression panel of biomarkers (US 20100267574).
The network clearly shows meaningful relationships between the TFs and miRNAs in lung cancer. The interactions show that the tumor suppressor miRNAs (miR-29a, miR-16, miR-125, and let-7) that could target the oncogene HMGA1 are downregulated. Upregulation of HMGA1 induces expression of oncogenic miR-122. Another two pro-oncogenic miRNAs that can also target HMGA1, miR-196a-2 and miR-155, are upregulated in lung cancers [66, 67]. We observed that HMGA1 may inhibit the putative tumor-suppressor IRF1 (as per the interaction network) and that the miR-155 pro-oncomiR directly targeted IRF1. Therefore, in this network, HMGA1 is the key TF that positively regulates lung tumorigenesis through upregulation of miR-122 and perhaps by downregulation of IRF1. However, we found that IRF1 is upregulated in the samples so that the IRF1-HMGA1 interactions need further attention.
Tumor suppressor RBL1 is a target of the miR-17 oncomiR . Furthermore, as per the interaction network, RBL1 is activated by TAF1 and cMYC, and regulates expression of E2F2, RB1, MCM7, and TFDP2. It thereby regulates the cell cycle and cell proliferation. Therefore, RBL1 downregulation and upregulation of miR-17 provide a meaningful mechanism in lung cancer tumorigenesis [66, 69].
The common pathway (of both NSCLC and SCLC) related genes HNRPD, E2F6, TFDP1, and SUV39H1 also showed the expected TF-miRNA relationship in the interaction map represented in Figure 6 based on the available experimental evidence. The literature shows that HNRPD and SUV39H1 may have positive roles in tumorigenesis [55, 56, 64]. Although in our blood-based qPCR, HNRPD and SUV39H1 are downregulated, they are reported to be upregulated in a mouse model of lung cancer , consistent with the tissue-based microarray analysis in our lung cancer samples. The involvement of HNRPD and SUV39H1 is further supported by reports that the tumor suppressor miR-125 is downregulated in both NSCLC and SCLC [70, 71]. Furthermore, the tumor suppressor protein RB1 is downregulated in lung cancer  and may inhibit SUV39H1.
The other two markers, E2F6 and TFDP1, are upregulated in all of our blood samples. While two pro-oncogenic miRNAs, miR-28 and miR-193, are upregulated  the putative tumor-suppressor, miR-137, is downregulated in lung cancers [72, 73]. All three of these miRNAs target E2F6 [74, 75]. Furthermore, E2F6 putatively upregulates TFDP1 and is downregulated by RB1. It is also found from the interaction map that E2F6 inhibition by two upregulated pro-oncomiRs (miR-28 and miR-193) is not sufficient, as the E2F6 was found to be upregulated in lung cancer. Further, E2F6 has been reported to upregulate oncogene TFDP1 and to positively regulate cell proliferation and cell survival through E2F1 . Additionally, downregulation of RB1 in lung cancer is not able to repress TFDP1 activity, and therefore, in lung cancer, tumorigenesis is mediated through upregulation of E2F6 and TFDP1. However, the role of SUV39H1 and HNRPD requires further exploration.
In this analysis, using an integrated reverse-transcriptomics-based bioinformatics approach, we have identified key transcription factors that may be useful in developing subtype specific biomarkers in lung cancer. Our proposed seven markers also have high potential to be used in lung cancer diagnostics for NSCLC subtypes. Of course, additional experimental validation in independent sets of patients is required to establish the diagnostic accuracy of this panel and we are currently conducting those experiments. The miRNA-TF-miRNA relationships with these seven miRNAs show meaningful associations with these TFs in lung cancer pathogenesis. The novel strategy developed in this research is powerful and can be applicable to identify molecular mechanisms and markers in other cancers as well.
This work was carried out without any grant. VA had funding from CNPq and FAPEMIG.
Conflict of interest
Authors declare no conflict of interest.
Publication for this article has been funded by NSF 1158608.
This article has been published as part of BMC Genomics Volume 14 Supplement 6, 2013: Proceedings of the International Conference of the Brazilian Association for Bioinformatics and Computational Biology (X-meeting 2012). The full contents of the supplement are available online at http://www.biomedcentral.com/bmcgenomics/supplements/14/S6.
- Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D: Global cancer statistics. CA Cancer J Clin. 2011, 61 (2): 69-90. 10.3322/caac.20107.PubMedView ArticleGoogle Scholar
- Petersen I: The morphological and molecular diagnosis of lung cancer. Dtsch Arztebl Int. 2011, 108 (31-32): 525-531.PubMedPubMed CentralGoogle Scholar
- Wang T, Nelson RA, Bogardus A, Grannis FW: Five-year lung cancer survival: which advanced stage nonsmall cell lung cancer patients attain long-term survival?. Cancer. 2010, 116 (6): 1518-1525. 10.1002/cncr.24871.PubMedView ArticleGoogle Scholar
- Pastorino U: Lung cancer screening. Br J Cancer. 2010, 102 (12): 1681-1686. 10.1038/sj.bjc.6605660.PubMedPubMed CentralView ArticleGoogle Scholar
- Granville CA, Dennis PA: An overview of lung cancer genomics and proteomics. Am J Respir Cell Mol Biol. 2005, 32 (3): 169-176. 10.1165/rcmb.F290.PubMedView ArticleGoogle Scholar
- Bianchi F, Nicassio F, Marzi M, Belloni E, Dall'olio V, Bernard L, Pelosi G, Maisonneuve P, Veronesi G, Di Fiore PP: A serum circulating miRNA diagnostic test to identify asymptomatic high-risk individuals with early stage lung cancer. EMBO Mol Med. 2011, 3 (8): 495-503. 10.1002/emmm.201100154.PubMedPubMed CentralView ArticleGoogle Scholar
- Hennessey PT, Sanford T, Choudhary A, Mydlarz WW, Brown D, Adai AT, Ochs MF, Ahrendt SA, Mambo E, Califano JA: Serum microRNA Biomarkers for Detection of Non-Small Cell Lung Cancer. PLoS One. 2012, 7 (2): e32307-10.1371/journal.pone.0032307.PubMedPubMed CentralView ArticleGoogle Scholar
- Yu L, Todd NW, Xing L, Xie Y, Zhang H, Liu Z, Fang H, Zhang J, Katz RL, Jiang F: Early detection of lung adenocarcinoma in sputum by a panel of microRNA markers. Int J Cancer. 2010, 127 (12): 2870-2878. 10.1002/ijc.25289.PubMedPubMed CentralView ArticleGoogle Scholar
- Liao J, Yu L, Mei Y, Guarnera M, Shen J, Li R, Liu Z, Jiang F: Small nucleolar RNA signatures as biomarkers for non-small-cell lung cancer. Mol Cancer. 2010, 9: 198-10.1186/1476-4598-9-198.PubMedPubMed CentralView ArticleGoogle Scholar
- Xing L, Todd NW, Yu L, Fang H, Jiang F: Early detection of squamous cell lung cancer in sputum by a panel of microRNA markers. Mod Pathol. 2010, 23 (8): 1157-1164. 10.1038/modpathol.2010.111.PubMedView ArticleGoogle Scholar
- Guergova-Kuras M, Kurucz I, Hempel W, Tardieu N, Kádas J, Malderez-Bloes C, Jullien A, Kieffer Y, Hincapie M, Guttman A, Csánky E, Dezso B, Karger BL, Takács L: Discovery of lung cancer biomarkers by profiling the plasma proteome with monoclonal antibody libraries. Mol Cell Proteomics. 2011, 10 (12): M111.010298-10.1074/mcp.M111.010298.PubMedPubMed CentralView ArticleGoogle Scholar
- Tong BC, Harpole DH: Molecular markers for incidence, prognosis, and response to therapy. Surg Oncol Clin N Am. 2012, 21 (1): 161-75. 10.1016/j.soc.2011.09.007.PubMedView ArticleGoogle Scholar
- Xiao H, Zhang L, Zhou H, Lee JM, Garon EB, Wong DT: Proteomic analysis of human saliva from lung cancer patients using two-dimensional difference gel electrophoresis and mass spectrometry. Mol Cell Proteomics. 2012, 11 (2): M111.012112-10.1074/mcp.M111.012112.PubMedPubMed CentralView ArticleGoogle Scholar
- Begum S, Brait M, Dasgupta S, Ostrow KL, Zahurak M, Carvalho AL, Califano JA, Goodman SN, Westra WH, Hoque MO, Sidransky D: An epigenetic marker panel for detection of lung cancer using cell-free serum DNA. Clin Cancer Res. 2011, 17 (13): 4494-4503. 10.1158/1078-0432.CCR-10-3436.PubMedPubMed CentralView ArticleGoogle Scholar
- Jiang F, Todd NW, Li R, Zhang H, Fang H, Stass SA: A panel of sputum-based genomic marker for early detection of lung cancer. Cancer Prev Res (Phila). 2010, 3 (12): 1571-1578. 10.1158/1940-6207.CAPR-10-0128.View ArticleGoogle Scholar
- Showe MK, Vachani A, Kossenkov AV, Yousef M, Nichols C, Nikonova EV, Chang C, Kucharczuk J, Tran B, Wakeam E, Yie TA, Speicher D, Rom WN, Albelda S, Showe LC: Gene expression profiles in peripheral blood mononuclear cells can distinguish patients with non-small cell lung cancer from patients with nonmalignant lung disease. Cancer Res. 2009, 69 (24): 9202-9210. 10.1158/0008-5472.CAN-09-1378.PubMedPubMed CentralView ArticleGoogle Scholar
- Barh D, Bhat D, Viero C: miReg: a resource for microRNA regulation. J Integr Bioinform. 2010, 7 (1): 144-Google Scholar
- Blenkiron C, Miska EA: miRNAs in cancer: approaches, aetiology, diagnostics and therapy. Hum Mol Genet. 2007, 16 (1): R106-13.PubMedView ArticleGoogle Scholar
- Sempere LF, Liu X, Dmitrovsky E: Tumor-suppressive microRNAs in Lung cancer: diagnostic and therapeutic opportunities. Scientific World Journal. 2009, 9: 626-628.PubMedPubMed CentralView ArticleGoogle Scholar
- Barshack I, Lithwick-Yanai G, Afek A, Rosenblatt K, Tabibian-Keissar H, Zepeniuk M, Cohen L, Dan H, Zion O, Strenov Y, Polak-Charcon S, Perelman M: MicroRNA expression differentiates between primary lung tumors and metastases to the lung. Pathol Res Pract. 2010, 206 (8): 578-584. 10.1016/j.prp.2010.03.005.PubMedView ArticleGoogle Scholar
- Hu Z, Chen X, Zhao Y, Tian T, Jin G, Shu Y, Chen Y, Xu L, Zen K, Zhang C, Shen H: Serum microRNA signatures identified in a genome-wide serum microRNA expression profiling predict survival of non-small-cell lung cancer. J Clin Oncol. 2010, 28 (10): 1721-1726. 10.1200/JCO.2009.24.9342.PubMedView ArticleGoogle Scholar
- Esquela-Kerscher A, Slack FJ: Oncomirs - microRNAs with a role in cancer. Nat Rev Cancer. 2006, 6 (4): 259-269. 10.1038/nrc1840.PubMedView ArticleGoogle Scholar
- Jiang Q, Wang Y, Hao Y, Juan L, Teng M, Zhang X, Li M, Wang G, Liu Y: miR2Disease: a manually curated database for microRNA deregulation in human disease. Nucleic Acids Res. 2009, 37 (Database): D98-104. 10.1093/nar/gkn714.PubMedPubMed CentralView ArticleGoogle Scholar
- Tsou JA, Galler JS, Siegmund KD, Laird PW, Turla S, Cozen W, Hagen JA, Koss MN, Laird-Offringa IA: Identification of a panel of sensitive and specific DNA methylation markers for lung adenocarcinoma. Mol Cancer. 2007, 6: 70-PubMedPubMed CentralView ArticleGoogle Scholar
- Dweep H, Sticht C, Pandey P, Gretz N: miRWalk--database: prediction of possible miRNA binding sites by "walking" the genes of three genomes. J Biomed Inform. 2011, 44 (5): 839-47. 10.1016/j.jbi.2011.05.002.PubMedView ArticleGoogle Scholar
- Xiao F, Zuo Z, Cai G, Kang S, Gao X, Li T: miRecords: an integrated resource for microRNA-target interactions. Nucleic Acids Res. 2009, 37 (Database): D105-10. 10.1093/nar/gkn851.PubMedPubMed CentralView ArticleGoogle Scholar
- Hsu SD, Lin FM, Wu WY, Liang C, Huang WC, Chan WL, Tsai WT, Chen GZ, Lee CJ, Chiu CM, Chien CH, Wu MC, Huang CY, Tsou AP, Huang HD: miRTarBase: a database curates experimentally validated microRNA-target interactions. Nucleic Acids Res. 2011, 39 (Database): D163-9. 10.1093/nar/gkq1107.PubMedPubMed CentralView ArticleGoogle Scholar
- Chen J, Bardes EE, Aronow BJ, Jegga AG: ToppGene Suite for gene list enrichment analysis and candidate gene prioritization. Nucleic Acids Res. 2009, 37 (Web Server): W305-11. 10.1093/nar/gkp427.PubMedPubMed CentralView ArticleGoogle Scholar
- Huang DW, Sherman BT, Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID Bioinformatics Resources. Nature Protoc. 2009, 4 (1): 44-57.View ArticleGoogle Scholar
- Breitkreutz BJ, Stark C, Tyers M: Osprey: a network visualization system. Genome Biol. 2003, 4 (3): R22-10.1186/gb-2003-4-3-r22.PubMedPubMed CentralView ArticleGoogle Scholar
- Wang J, Lu M, Qiu C, Cui Q: TransmiR: a transcription factor-microRNA regulation database. Nucleic Acids Res. 2010, 38 (Database): D119-22. 10.1093/nar/gkp803.PubMedPubMed CentralView ArticleGoogle Scholar
- Friard O, Re A, Taverna D, De Bortoli M, Corá D: CircuitsDB: a database of mixed microRNA/transcription factor feed-forward regulatory circuits in human and mouse. BMC Bioinformatics. 2010, 11: 435-10.1186/1471-2105-11-435.PubMedPubMed CentralView ArticleGoogle Scholar
- Hu Z, Hung JH, Wang Y, Chang YC, Huang CL, Huyck M, DeLisi C: VisANT 3.5: multi-scale network visualization, analysis and inference based on the gene ontology. Nucleic Acids Res. 2009, 37 (Web Server): W115-21. 10.1093/nar/gkp406.PubMedPubMed CentralView ArticleGoogle Scholar
- Akaboshi S, Watanabe S, Hino Y, Sekita Y, Xi Y, Araki K, Yamamura K, Oshima M, Ito T, Baba H, Nakao M: HMGA1 is induced by Wnt/beta-catenin pathway and maintains cell proliferation in gastric cancer. Am J Pathol. 2009, 175 (4): 1675-1685. 10.2353/ajpath.2009.090069.PubMedPubMed CentralView ArticleGoogle Scholar
- Belton A, Gabrovsky A, Bae YK, Reeves R, Iacobuzio-Donahue C, Huso DL, Resar LM: HMGA1 induces intestinal polyposis in transgenic mice and drives tumor progression and stem cell properties in colon cancer cells. PLoS One. 2012, 7 (1): e30034-10.1371/journal.pone.0030034.PubMedPubMed CentralView ArticleGoogle Scholar
- Pang B, Fan H, Zhang IY, Liu B, Feng B, Meng L, Zhang R, Sadeghi S, Guo H, Pang Q: HMGA1 expression in human gliomas and its correlation with tumor proliferation, invasion and angiogenesis. J Neurooncol. 2012, 106 (3): 543-549. 10.1007/s11060-011-0710-6.PubMedView ArticleGoogle Scholar
- Hillion J, Wood LJ, Mukherjee M, Bhattacharya R, Di Cello F, Kowalski J, Elbahloul O, Segal J, Poirier J, Rudin CM, Dhara S, Belton A, Joseph B, Zucker S, Resar LM: Upregulation of MMP-2 by HMGA1 promotes transformation in undifferentiated, large-cell lung cancer. Mol Cancer Res. 2009, 7 (11): 1803-1812. 10.1158/1541-7786.MCR-08-0336.PubMedPubMed CentralView ArticleGoogle Scholar
- Sarhadi VK, Wikman H, Salmenkivi K, Kuosma E, Sioris T, Salo J, Karjalainen A, Knuutila S, Anttila S: Increased expression of high mobility group A proteins in lung cancer. J Pathol. 2006, 209 (2): 206-212. 10.1002/path.1960.PubMedView ArticleGoogle Scholar
- Scrima M, De Marco C, Fabiani F, Franco R, Pirozzi G, Rocco G, Ravo M, Weisz A, Zoppoli P, Ceccarelli M, Botti G, Malanga D, Viglietto G: Signaling networks associated with AKT activation in non-small cell lung cancer (NSCLC): new insights on the role of phosphatydil-inositol-3 kinase. PLoS One. 2012, 7 (2): e30427-10.1371/journal.pone.0030427.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhang Y, Ma T, Yang S, Xia M, Xu J, An H, Yang Y, Li S: High-mobility group A1 proteins enhance the expression of the oncogenic miR-222 in lung cancer cells. Mol Cell Biochem. 2011, 357 (1-2): 363-371. 10.1007/s11010-011-0907-1.PubMedView ArticleGoogle Scholar
- Shan B, Farmer AA, Lee WH: The molecular basis of E2F-1/DP-1-induced S-phase entry and apoptosis. Cell Growth Differ. 1996, 7 (6): 689-697.PubMedGoogle Scholar
- Abba MC, Fabris VT, Hu Y, Kittrell FS, Cai WW, Donehower LA, Sahin A, Medina D, Aldaz CM: Identification of novel amplification gene targets in mouse and human breast cancer at a syntenic cluster mapping to mouse ch8A1 and human ch13q34. Cancer Res. 2007, 67 (9): 4104-4112. 10.1158/0008-5472.CAN-06-4672.PubMedPubMed CentralView ArticleGoogle Scholar
- Yasui K, Okamoto H, Arii S, Inazawa J: Association of over-expressed TFDP1 with progression of hepatocellular carcinomas. J Hum Genet. 2003, 48 (12): 609-613. 10.1007/s10038-003-0086-3.PubMedView ArticleGoogle Scholar
- Castillo SD, Angulo B, Suarez-Gauthier A, Melchor L, Medina PP, Sanchez-Verde L, Torres-Lanzas J, Pita G, Benitez J, Sanchez-Cespedes M: Gene amplification of the transcription factor DP1 and CTNND1 in human lung cancer. J Pathol. 2010, 222 (1): 89-98.PubMedGoogle Scholar
- Lu K, Shih C, Teicher BA: Expression of pRB, cyclin/cyclin-dependent kinases and E2F1/DP-1 in human tumor lines in cell culture and in xenograft tissues and response to cell cycle agents. Cancer Chemother Pharmacol. 2000, 46 (4): 293-304. 10.1007/s002800000136.PubMedView ArticleGoogle Scholar
- Usuda J, Saijo N, Fukuoka K, Fukumoto H, Kuh HJ, Nakamura T, Koh Y, Suzuki T, Koizumi F, Tamura T, Kato H, Nishio K: Molecular determinants of UCN-01-induced growth inhibition in human lung cancer cells. Int J Cancer. 2000, 85 (2): 275-280. 10.1002/(SICI)1097-0215(20000115)85:2<275::AID-IJC20>3.0.CO;2-5.PubMedView ArticleGoogle Scholar
- Nozawa H, Oda E, Ueda S, Tamura G, Maesawa C, Muto T, Taniguchi T, Tanaka N: Functionally inactivating point mutation in the tumor-suppressor IRF-1 gene identified in human gastric cancer. Int J Cancer. 1998, 77 (4): 522-7. 10.1002/(SICI)1097-0215(19980812)77:4<522::AID-IJC8>3.0.CO;2-W.PubMedView ArticleGoogle Scholar
- Cavalli LR, Riggins RB, Wang A, Clarke R, Haddad BR: Frequent loss of heterozygosity at the interferon regulatory factor-1 gene locus in breast cancer. Breast Cancer Res Treat. 2010, 121 (1): 227-231. 10.1007/s10549-009-0509-8.PubMedPubMed CentralView ArticleGoogle Scholar
- Wenzel J, Tomiuk S, Zahn S, Küsters D, Vahsen A, Wiechert A, Mikus S, Birth M, Scheler M, von Bubnoff D, Baron JM, Merk HF, Mauch C, Krieg T, Bieber T, Bosio A, Hofmann K, Tüting T, Peters B: Transcriptional profiling identifies an interferon-associated host immune response in invasive squamous cell carcinoma of the skin. Int J Cancer. 2008, 123 (11): 2605-15. 10.1002/ijc.23799.PubMedView ArticleGoogle Scholar
- Gaubatz S, Wood JG, Livingston DM: Unusual proliferation arrest and transcriptional control properties of a newly discovered E2F family member, E2F-6. Proc Natl Acad Sci USA. 1998, 95 (16): 9190-9195. 10.1073/pnas.95.16.9190.PubMedPubMed CentralView ArticleGoogle Scholar
- Yang WW, Shu B, Zhu Y, Yang HT: E2F6 inhibits cobalt chloride-mimetic hypoxia-induced apoptosis through E2F1. Mol Biol Cell. 2008, 19 (9): 3691-3700. 10.1091/mbc.E08-02-0171.PubMedPubMed CentralView ArticleGoogle Scholar
- Villeneuve LM, Reddy MA, Lanting LL, Wang M, Meng L, Natarajan R: Epigenetic histone H3 lysine 9 methylation in metabolic memory and inflammatory phenotype of vascular smooth muscle cells in diabetes. Proc Natl Acad Sci USA. 2008, 105 (26): 9047-9052. 10.1073/pnas.0803623105.PubMedPubMed CentralView ArticleGoogle Scholar
- Yang YJ, Han JW, Youn HD, Cho EJ: The tumor suppressor, parafibromin, mediates histone H3 K9 methylation for cyclin D1 repression. Nucleic Acids Res. 2010, 38 (2): 382-90. 10.1093/nar/gkp991.PubMedPubMed CentralView ArticleGoogle Scholar
- Yokoyama Y, Hieda M, Nishioka Y, Matsumoto A, Higashi S, Kimura H, Yamamoto H, Mori M, Matsuura S, Matsuura N: Cancer associated up-regulation of H3K9 trimethylation promotes cell motility in vitro and drives tumor formation in vivo. Cancer Sci. 2013, doi: 10.1111/cas.12166Google Scholar
- Watanabe H, Soejima K, Yasuda H, Kawada I, Nakachi I, Yoda S, Naoki K, Ishizaka A: Deregulation of histone lysine methyltransferases contributes to oncogenic transformation of human bronchoepithelial cells. Cancer Cell Int. 2008, 8: 15-10.1186/1475-2867-8-15.PubMedPubMed CentralView ArticleGoogle Scholar
- Chen JH, Yeh KT, Yang YM, Chang JG, Lee HE, Hung SY: High expressions of histone methylation- and phosphorylation-related proteins are associated with prognosis of oral squamous cell carcinoma in male population of Taiwan. Med Oncol. 2013, 30 (2): 513-PubMedView ArticleGoogle Scholar
- Patani N, Jiang WG, Newbold RF, Mokbel K: Histone-modifier gene expression profiles are associated with pathological and clinical outcomes in human breast cancer. Anticancer Res. 2011, 31 (12): 4115-25.PubMedGoogle Scholar
- Zhu L, van den Heuvel S, Helin K, Fattaey A, Ewen M, Livingston D, Dyson N, Harlow E: Inhibition of cell proliferation by p107, a relative of the retinoblastoma protein. Genes Dev. 1993, 7 (7A): 1111-1125. 10.1101/gad.7.7a.1111.PubMedView ArticleGoogle Scholar
- Paramio JM, Laín S, Segrelles C, Lane EB, Jorcano JL: Differential expression and functionally co-operative roles for the retinoblastoma family of proteins in epidermal differentiation. Oncogene. 1998, 17 (8): 949-957. 10.1038/sj.onc.1202031.PubMedView ArticleGoogle Scholar
- Baldi A, Esposito V, De Luca A, Howard CM, Mazzarella G, Baldi F, Caputi M, Giordano A: Differential expression of the retinoblastoma gene family members pRb/p105, p107, and pRb2/p130 in lung cancer. Clin Cancer Res. 1996, 2 (7): 1239-1245.PubMedGoogle Scholar
- Zucconi BE, Wilson GM: Modulation of neoplastic gene regulatory pathways by the RNA-binding factor AUF1. Front Biosci. 2011, 16: 2307-2325. 10.2741/3855.View ArticleGoogle Scholar
- Shchors K, Yehiely F, Kular RK, Kotlo KU, Brewer G, Deiss LP: Cell death inhibiting RNA (CDIR) derived from a 3'-untranslated region binds AUF1 and heat shock protein 27. J Biol Chem. 2002, 277 (49): 47061-47072. 10.1074/jbc.M202272200.PubMedView ArticleGoogle Scholar
- Frau M, Tomasi ML, Simile MM, Demartis MI, Salis F, Latte G, Calvisi DF, Seddaiu MA, Daino L, Feo CF, Brozzetti S, Solinas G, Yamashita S, Ushijima T, Feo F, Pascale RM: Role of transcriptional and posttranscriptional regulation of methionine adenosyltransferases in liver cancer progression. Hepatology. 2012, 56 (1): 165-175. 10.1002/hep.25643.PubMedView ArticleGoogle Scholar
- Blaxall BC, Dwyer-Nield LD, Bauer AK, Bohlmeyer TJ, Malkinson AM, Port JD: Differential expression and localization of the mRNA binding proteins, AU-rich element mRNA binding protein (AUF1) and Hu antigen R (HuR), in neoplastic lung tissue. Mol Carcinog. 2000, 28 (2): 76-83. 10.1002/1098-2744(200006)28:2<76::AID-MC3>3.0.CO;2-0.PubMedView ArticleGoogle Scholar
- Xiaowei W: miRDB: a microRNA target prediction and functional annotation database with a wiki interface. RNA. 2008, 14 (6): 1012-1017. 10.1261/rna.965408.View ArticleGoogle Scholar
- Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, Petrocca F, Visone R, Iorio M, Roldo C, Ferracin M, Prueitt RL, Yanaihara N, Lanza G, Scarpa A, Vecchione A, Negrini M, Harris CC, Croce CM: A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci USA. 2006, 103 (7): 2257-2261. 10.1073/pnas.0510565103.PubMedPubMed CentralView ArticleGoogle Scholar
- Yanaihara N, Caplen N, Bowman E, Seike M, Kumamoto K, Yi M, Stephens RM, Okamoto A, Yokota J, Tanaka T, Calin GA, Liu CG, Croce CM, Harris CC: Unique microRNA molecular profiles in lung cancer diagnosis and prognosis. Cancer Cell. 2006, 9 (3): 189-198. 10.1016/j.ccr.2006.01.025.PubMedView ArticleGoogle Scholar
- Trompeter HI, Abbad H, Iwaniuk KM, Hafner M, Renwick N, Tuschl T, Schira J, Müller HW, Wernet P: MicroRNAs MiR-17, MiR-20a, and MiR-106b act in concert to modulate E2F activity on cell cycle arrest during neuronal lineage differentiation of USSC. PLoS One. 2011, 6 (1): e16138-10.1371/journal.pone.0016138.PubMedPubMed CentralView ArticleGoogle Scholar
- Hayashita Y, Osada H, Tatematsu Y, Yamada H, Yanagisawa K, Tomida S, Yatabe Y, Kawahara K, Sekido Y, Takahashi T: A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation. Cancer Res. 2005, 65 (21): 9628-9632. 10.1158/0008-5472.CAN-05-2352.PubMedView ArticleGoogle Scholar
- Miko E, Czimmerer Z, Csánky E, Boros G, Buslig J, Dezso B, Scholtz B: Differentially expressed microRNAs in small cell lung cancer. Exp Lung Res. 2009, 35 (8): 646-664. 10.3109/01902140902822312.PubMedView ArticleGoogle Scholar
- Raponi M, Dossey L, Jatkoe T, Wu X, Chen G, Fan H, Beer DG: MicroRNA classifiers for predicting prognosis of squamous cell lung cancer. Cancer Res. 2009, 69 (14): 5776-5783. 10.1158/0008-5472.CAN-09-0587.PubMedView ArticleGoogle Scholar
- Yu SL, Chen HY, Chang GC, Chen CY, Chen HW, Singh S, Cheng CL, Yu CJ, Lee YC, Chen HS, Su TJ, Chiang CC, Li HN, Hong QS, Su HY, Chen CC, Chen WJ, Liu CC, Chan WK, Chen WJ, Li KC, Chen JJ, Yang PC: MicroRNA signature predicts survival and relapse in lung cancer. Cancer Cell. 2008, 13 (1): 48-57. 10.1016/j.ccr.2007.12.008.PubMedView ArticleGoogle Scholar
- Dacic S, Kelly L, Shuai Y, Nikiforova MN: miRNA expression profiling of lung adenocarcinomas: correlation with mutational status. Mod Pathol. 2010, 23 (12): 1577-1582. 10.1038/modpathol.2010.152.PubMedView ArticleGoogle Scholar
- Girardot M, Pecquet C, Boukour S, Knoops L, Ferrant A, Vainchenker W, Giraudier S, Constantinescu SN: miR-28 is a thrombopoietin receptor targeting microRNA detected in a fraction of myeloproliferative neoplasm patient platelets. Blood. 2010, 116 (3): 437-445. 10.1182/blood-2008-06-165985.PubMedView ArticleGoogle Scholar
- Kozaki K, Imoto I, Mogi S, Omura K, Inazawa J: Exploration of tumor-suppressive microRNAs silenced by DNA hypermethylation in oral cancer. Cancer Res. 2008, 68 (7): 2094-2105. 10.1158/0008-5472.CAN-07-5194.PubMedView ArticleGoogle Scholar
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