Substrate-dependent gene regulation of self-assembled human MSC spheroids on chitosan membranes
© Yeh et al.; licensee BioMed Central Ltd. 2014
Received: 18 April 2013
Accepted: 21 December 2013
Published: 5 January 2014
Three-dimensional (3D) multicellular spheroids of mesenchymal stem cells (MSCs) are generally regarded to have beneficial properties over MSCs in monolayer. Recent literatures have documented that MSCs can self-assemble into 3D spheroids with a greater capacity for differentiation into various cell types when grown on chitosan (CS), a biopolymer. The genomic modulation occurring in these MSC spheroids is thus of essential importance for understanding their uniqueness and therapeutic potentials. In this study, 3D spheroids self-assembled from human umbilical cord MSCs grown on CS membranes were analyzed by mRNA as well as microRNA microarrays, which helped identify the critical signaling events that may alter the cellular functions during the spheroid forming process.
Genes screened from mRNA and microRNA cross-correlation analyses were further confirmed with the quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis. Results revealed the regulation of a significant number of calcium-associated genes, which suggested the crucial role of calcium signaling in CS-derived MSC spheroids. In addition, many genes associated with the multilineage differentiation capacities and those associated with the antiinflammatory and antitumor properties of MSCs were upregulated. The genetic modulation was significantly more remarkable and endured longer for MSC spheroids derived on CS substrates compared to those derived on a non-adherent (polyvinyl alcohol) substrate.
Based on the study, the culture substrates used to prepare 3D MSC spheroids may predefine their properties through cell-substrate interaction.
KeywordsMesenchymal stem cells (MSCs) Cellular spheroids Chitosan Calcium signaling Gene profile Microarray
Mesenchymal stem cells (MSCs) are extensively used as the cell source for regenerative medicine because of their capacities to differentiate into different lineages and expand while maintaining their undifferentiated state. MSCs are commonly cultured as two-dimensional (2D) monolayer by traditional tissue culture techniques. However, the 2D culture method has difficulty in overcoming the replicative senescence and maintaining the self-renewal properties, which results in cells of low differentiation capacity . A three-dimensional (3D) culture environment is considered more favorable than 2D monolayer culture for cell-cell contacts. Previous studies have developed several methods to generate 3D MSC spheroids. Many of these methods involve the use of cell suspension system or non-adherent surface to induce spheroid formation [2–4]. In general, these 3D MSC spheroids were reported to have greater differentiation capacities.
Chitosan (CS) is the deacetylated derivative of chitin which is abundant in shell of shrimp or crap, and in fungus, and the content is only next to cellulose in nature. CS has a main structure composed of β(1-4) linked D-glucosamine residues with different amounts of N-acetyl-glucosamine group. Owing to its biocompatibility and biodegradability, CS has been widely studied as a scaffolding material for tissue engineering. Recent findings have revealed that MSCs attached on the membranes made of CS can form self-assembled 3D cellular spheroids [5–7]. During the process, MSCs attach and spread on CS membranes before they retract their pseudopodia to form multicellular spheroids. This self-assembly process is quite different from that occurs in suspension or hanging drop systems, or on non-adherent polymer surfaces. Several genes/proteins have been referred to participate in the process of spheroid formation on CS, including cadherin molecules [8, 9], Rho/Rho-associated kinase (ROCK) , and the Wnt molecule . Activations of these proteins were not as evident for spheroids on non-adherent surfaces. In addition, it was observed that the surface-bound calcium on CS substrates may be transported into MSCs and play a role in spheroid formation as well as gene regulation . Although a few changes in gene/protein expression were observed, the exact mechanism for spheroid formation on CS is still far from being elucidated. Therefore, a more comprehensive understanding of the genomic profile for CS-derived MSC spheroids is essential for further revealing the substrate-dependent nature of these unique MSC spheroids.
The technique of microarray has been developed to detect the changes within cells and is a powerful tool by which many genes can be probed simultaneously. Dalby et al. have reported the genomic expression profile of human MSCs responding to the shape of their environment by the messenger RNA (mRNA) microarray . The antiinflammatory properties of human MSC spheroids generated by hanging drop have also been compared to those of the adherent MSC monolayer by surveying with mRNA microarray . Furthermore, recent advances in microRNA (miRNA), a class of non-coding small RNA, have identified a few important modulators in stem cell proliferation and differentiation. They can bind to the cognate mRNA to repress the expression of target genes. Simultaneous analyses of the mRNA and miRNA expression profiles may help narrowing down the signaling events involved in the behavior change of the cells .
In this study, we examined both mRNA and miRNA expression profiles of the CS substrate-induced 3D spheroids of human MSCs isolated from the umbilical cord, using 2D MSCs on tissue culture polystyrene (TCPS) as a control. Cross-correlation analysis of the results from these two microarrays was further confirmed with the quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis to identify the critical signaling events for substrate-derived MSC spheroids during the spheroid forming process.
Characteristics of human umbilical cord MSCs
The expression profile of cell surface markers analyzed by flow cytometry is shown in Additional file 1: Figure S1. Human umbilical cord MSCs were positive for specific antigen markers of MSCs such as CD13, CD29, CD44, CD59, CD61, CD71, CD73, CD90, CD105, CD166, and HLA-ABC, and negative for specific markers of endothelial cells and haematopoietic cells including CD14, CD34, CD45, CD133, and HLA-DR. Besides, cells showed positive expression of CD56 and low expression of CD106, which was consistent with that described for human umbilical cord MSCs in literatures [12, 13].
Surface properties of CS membranes
Spheroid formation for MSCs growth on CS membranes
The morphology of MSCs grown on CS membranes and TCPS is shown in Figure 2. As was expected, MSCs attached to TCPS with fibroblast-like morphology. On the other hand, MSCs on CS remained attached to the surface before 16 h and formed spheroids afterwards. The average diameter of the MSC spheroids on CS membranes was 64.9 ± 9.5 μm at 24 h (n = 30). The spheroid size was relatively stable within 72 h (e.g. averaged 56.9 ± 14.3 μm at 72 h, n = 30). The cell viability for the MSC spheroids was 93.11% at 24 h.
Cross-correlation analysis between mRNA and miRNA microarrays
The biological significance behind the list of screened genes was further analyzed by the DAVID (the Database for Annotation, Visualization and Integrated Discovery) program to evaluate the gene enrichment in gene ontology (GO terms) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway maps (Additional file 3). The results of gene enrichment in KEGG pathway maps suggested that several pathways were regulated for MSCs on CS vs. TCPS including calcium signaling pathway (hsa04020), focal adhesion (hsa04510), regulation of actin cytoskeleton (hsa04810), adherens junction (hsa04520), gap junction (hsa04540), ECM-receptor interaction (hsa04512), p53 signaling pathway (hsa04115), cytokine-cytokine receptor interaction (hsa04060), tryptophan metabolism (hsa00380), and TGF-β signaling pathway (hsa04350).
Among the 285 genes, we picked up the ones with high expression ratio, the critical ones in certain signaling pathways, or the ones which had been reported in literature to be associated with cell adhesion, migration, or fate decision. They were then further confirmed by qRT-PCR.
Regulation of calcium-associated genes for MSCs on CS
The group of calcium signaling-associated genes screened from microarrays
Gene full name
ATPase, Ca++ transporting, plasma membrane 1
3.17 ± 0.88*
ATPase, Ca++ transporting, plasma membrane 4
0.68 ± 0.23*
solute carrier family 8 (sodium/calcium exchanger), member 1
0.40 ± 0.15*
transient receptor potential cation channel, subfamily A, member 1
24.58 ± 16.15*
transient receptor potential cation channel, subfamily C, member 4
0.02 ± 0.01*
5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled
26.92 ± 10.07*
5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled
platelet-derived growth factor receptor, alpha polypeptide
3.38 ± 1.17*
G protein-coupled receptor 68
10.80 ± 1.42*
coagulation factor II (thrombin) receptor
6.98 ± 1.53*
bradykinin receptor B2
endothelin receptor type A
adrenoceptor beta 2, surface
mitogen-activated protein kinase kinase kinase 8
13.31 ± 2.04*
inositol 1,4,5-trisphosphate receptor, type 1
2.36 ± 0.32*
phospholipase A2, group IVA (cytosolic, calcium-dependent)
6.44 ± 2.70*
RAS guanyl releasing protein 3 (calcium and DAG-regulated)
2.78 ± 0.90*
calmodulin 2 (phosphorylase kinase, delta)
0.08 ± 0.05*
A few genes of calcium-associated receptors were upregulated for MSCs growth on CS vs. TCPS. HTR2A and HTR7 (5-hydroxytryptamine receptor 2 A and 7) are the receptors for serotonin which is a well-characterized neurotransmitter with regulative function in multiple physiological aspects . GPR68 (G protein-coupled receptor 68, also named as OGR1) is a proton-sensing receptor that can modulate the level of intracellular calcium . PDGFRA (Platelet-derived growth factor receptor alpha) and F2R (thrombin-activated G protein-coupled receptor, and often named as the protease-activated receptor-1, PAR1) are also intracellular calcium modulators [21, 22]. The activation of these calcium-associated receptors may directly enhance the level of intracellular calcium and lead further to cytoskeleton rearrangement.
Several intracellular signaling genes were also upregulated. These genes included those of MAP3k8 (mitogen-activated protein kinase kinase kinase 8, also denoted tumor progression locus 2, Tpl2), ITPR1( inositol 1,4,5-trisphosphate receptor, type 1), RASGRP3 (calcium and diacylglycerol-regulated RAS guanyl releasing protein 3), PLA2G4A (cytosolic calcium-dependent phospholipase A2). MAP3K8 is required for the transduction of signals initiated by PAR1 and other G-coupled receptors, which promote actin reorganization and cell migration. MAP3K8 can also mediate signal-induced increases in cytoplasmic Ca2+ through the activation of phospholipase C . On the other hand, ITPR1 is a Ca2+-release channel located on intracellular membranes, especially the endoplasmic reticulum (ER). The IP3 receptor has an affinity for IP3 in the low nanomolar range. Moreover, cytosolic Ca2+ is considered as a co-agonist of the IP3 receptor, as it strongly increases the IP3 receptor activity at concentrations up to about 300 nM . RASGRP3 is a calcium and DAG-regulated RAS guanyl releasing protein which can activate small GTPases such as RAS and RAP1 . PLA2G4A is a member of phospholipases A2 (PLA2s) superfamily, which regulates the release of arachidonic acid (AA) . Although calmodulin is also a family of Ca2+ binding proteins and mediates many important cellular processes , CALM2 (calmodulin 2) gene was downregulated.
The regulation of various calcium-associated genes suggested the critical role of calcium signaling in the CS-derived MSC spheroids, which has not yet been reported in any other spheroid systems.
Regulation of cell adhesion and migration/cytoskeleton-associated genes for MSCs on CS
The group of adhesion and migration/cytoskeleton-associated genes screened from microarrays
Gene full name
integrin, beta 8
5.28 ± 2.30*
integrin, alpha 2
integrin, alpha 10
integrin, alpha 11
integrin, beta 1
integrin, alpha 6
matrix metallopeptidase 10 (stromelysin 2)
39.10 ± 12.35*
matrix metallopeptidase 1 (interstitial collagenase)
2.75 ± 0.72*
matrix metallopeptidase 3 (stromelysin 1, progelatinase)
cadherin 18, type 2
5.46 ± 3.08*
4.18 ± 1.70*
platelet/endothelial cell adhesion molecule 1
2.68 ± 1.04*
2.40 ± 0.43*
delta-like 1 (Drosophila)
2.27 ± 0.19*
EPH receptor A7
12.34 ± 4.95*
sarcoglycan, gamma (35 kDa dystrophin-associated glycoprotein)
SHC (Src homology 2 domain containing) family, member 4
protein tyrosine phosphatase, receptor type, B
sorbin and SH3 domain containing 2
7.11 ± 1.90*
0.38 ± 0.06*
collagen and calcium binding EGF domains 1
0.19 ± 0.07*
hyaluronan-mediated motility receptor (RHAMM)
0.29 ± 0.06*
chemokine-like receptor 1
93.69 ± 36.86*
chemokine (C-X-C motif) receptor 4
16.61 ± 4.90*
chemokine (C-X-C motif) receptor 7
10.78 ± 4.99*
chemokine (C-X-C motif) ligand 10
8.99 ± 4.86*
chemokine (C-C motif) ligand 2
chemokine (C-C motif) ligand 7
On the other hand, a variety of genes that controls cell-to-cell adhesion were upregulated for MSCs on CS, including cadherins, cell adhesion molecules (CAMs), Notch, and ephrin receptor. CDH18 (cadherin 18, also named as cadherin 14) is a Ca2+-dependent cell-cell adhesion molecule, and expresses in the central nervous system . PCDH18 (protocadherin 18) is also a member of cadherin family, and has a role in embryo development . PECAM1 (platelet endothelial cell adhesion molecule, also known as CD31) is an endothelial cell marker . The expression of NOTCH3 (Notch receptor 3) and its ligand, DLL1 (delta-like protein 1), were both enhanced for MSC growth on CS. Notch signaling pathway is critical for cell fate decisions including proliferation, lineage commitment, and terminal differentiation in many adult stem cell types . EphA7 (Ephrin type-A receptor 7) can bind to cell surface-associated ephrin ligands on neighboring cells to generate bidirectional signals that affect both the receptor-expressing and ephrin-expressing cells . Based on the literature, EphA7 is an axon guidance receptor important for the development of cortical circuits . The enhancement in these cell-cell adhesion genes may provide better cell-cell communication and coordination during spheroid formation.
The upregulation of chemokines and their receptors for MSCs grown on CS vs. TCPS is of particular interest. Among them, the gene encoding CMKLR1 (chemokine-like receptor 1, also as chemerin Receptor 23, ChemR23) was upregulated superbly (~50 times). CMKLR1 was reported as a multifunctional receptor which can bind with the proinflammatory chemokine, chemerin, or with the anti-inflammatory lipid mediator, resolving E1 (RvE1, a bioactive oxygenated product of the essential fatty acid, eicosapentaenoic acid) . Chemerin/CMKLR1 interaction was also reported to promote adipogenesis and angiogenesis . Other upregulated chemokine receptors or ligands included the CXCR4 and CXCR7 (CXC motif chemokine receptor 4 and 7), which are the receptors of stromal derived factor-1 (SDF1 or CXCL12). CXCR4 is one of the most studied chemokine receptors that play an important role in cell migration, proliferation, and differentiation . The CXCL10 (CXC motif chemokine ligand 10) is a ligand for another CXC motif chemokine receptor, CXCR3, which was reported to crosstalk with CXCR4 and CXCR7 . The CCL2 and CCL7 [C-C motif chemokine ligand 2 and 7, also referred as monocyte chemotactic protein 1 and 3 (MCP-1 and MCP-3)] are important homing factors for MSCs [38, 39].
Cell fate decision in MSC spheroids
The group of development-associated genes screened from microarrays
Gene full name
transforming growth factor, beta 3
2.55 ± 0.89*
bone morphogenetic protein 2
9.79 ± 3.33*
hepatocyte growth factor (hepapoietin A; scatter factor)
7.89 ± 2.73*
insulin-like growth factor 1 receptor
2.26 ± 0.71*
kinase insert domain receptor (a type III receptor tyrosine kinase)
5.30 ± 1.48*
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
6.21 ± 1.23*
epidermal growth factor
heparin-binding EGF-like growth factor
connective tissue growth factor
brain-derived neurotrophic factor
0.33 ± 0.10*
growth hormone receptor
3.95 ± 0.71*
lymphoid enhancer-binding factor 1
2.14 ± 0.16*
transcription factor 7 (T-cell specific, HMG-box)
1.35 ± 0.03*
dishevelled associated activator of morphogenesis 1
3.61 ± 0.60*
wingless-type MMTV integration site family member 2
4.66 ± 0.85*
low density lipoprotein receptor-related protein 4
diacylglycerol kinase, gamma 90 kDa
CXXC finger protein 4
7.21 ± 1.02*
retinoic acid receptor, beta
1.68 ± 0.16*
early growth response 2
25.32 ± 4.83*
Several WNT signaling related genes were also enhanced for MSCs on CS including the WLS (Wntless protein), WNT2 (wingless-type MMTV integration site family member 2), LEF1 (lymphoid enhancer-binding factor 1), TCF7 (transcription factor 7), DAAM1 (dishevelled associated activator of morphogenesis 1), and CXXC4 (CXXC finger protein 4). The WNT proteins and WNT signaling pathway are known to control cell specification and fate during embryonic development and adult tissue homeostasis. WNT2, a member of WNT family, can promote the earliest aspects of lung airway smooth muscle development , and accelerate cardiac myocyte differentiation from ES-cell derived mesodermal cells through the non-canonical WNT pathway . WLS is a multipass transmembrane protein, and was found to control the secretion of WNT proteins . The TCF/LEF family is the downstream proteins in the canonical WNT/ β-catenin pathway. In response to WNT signals, TCF/LEF members present as a switch to modulate the transcription of numerous target genes from repression to activation as binding with β-catenin . DAAM1 was identified as a downstream protein interacting with Dishevelled (Dvl), which mediates the non-canonical Wnt/planar cell polarity (PCP) signaling pathway. A study indicated that DAAM1 may play a crucial role in regulating the actin cytoskeleton and tissue morphogenesis . On the other hand, one of the negative regulators of WNT/ β-catenin signaling pathway, CXXC4, was also enhanced for MSCs on CS .
Two other development-related genes worth mentioning are RARB (retinoic acid receptor beta) and EGR2 (early growth response 2). RARB is a nuclear receptor for retinoic acid (RA) which is a vitamin A-derived, non-peptidic, small lipophilic molecule. The RA signaling during embryo development has been extensively investigated . EGR2 is a zinc-finger transcription factor of the early growth response gene (EGR) family that has critical functions in hindbrain development and myelination of the peripheral nervous system . The gene expression of EGR2 may be regulated by TGF-β3 .
Antiinflammatory and antitumor properties of MSCs on CS
The group of antiinflammatory and antitumor genes screened from microarrays
Gene full name
interleukin 1, alpha
3.48 ± 1.09*
interleukin 1, beta
interleukin 1 receptor antagonist
5.72 ± 0.88*
interleukin-1 receptor-associated kinase 2
interleukin 4 induced gene 1
18.59 ± 10.05*
interleukin 6 signal transducer (gp130, oncostatin M receptor)
leukemia inhibitory factor
3.70 ± 1.77*
tumor necrosis factor, alpha-induced protein 8-like 3
10.28 ± 2.41*
tumor necrosis factor, alpha-induced protein 8
7.02 ± 1.75*
tumor necrosis factor, alpha-induced protein 6
tumor necrosis factor (ligand) superfamily, member 13b
tumor necrosis factor (ligand) superfamily, member 9
tumor necrosis factor receptor superfamily, member 11b
C1q and tumor necrosis factor related protein 6
prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
15.09 ± 1.73*
tumor protein p53
1.48 ± 0.03*
A few antitumor genes were upregulated for MSCs on CS. Of interest to note is the high gene expression level of IL24 (interleukin 24) observed for MSCs grown on CS vs. TCPS. IL24 is considered as a tumor suppressor and can selectively induce apoptosis in cancer cells without affecting normal cells [59, 60]. TP53 ( tumor protein p53) is another upregulated tumor suppressor gene that involves in various critical cellular functions such as proliferation, cell cycle arrest, apoptosis, and DNA repair mechanisms . The members of TNFα-induced protein 8 (TNFAIP8) family were upregulated. TNFAIP8 was reported as an apoptosis regulator, while the gene encoding TNFAIP8L3 (TNFα-induced protein 8 like 3) is still unknown for its biological function .
Regulation of aryl hydrocarbon receptor (AHR) pathway
Other significant genes screened from microarrays
Gene full name
aryl hydrocarbon receptor
4.29 ± 1.33*
aryl-hydrocarbon receptor nuclear translocator 2
cytochrome P450, family 1, subfamily B, polypeptide 1
10.91 ± 0.57*
cytochrome P450, family 19, subfamily A, polypeptide 1
cytochrome P450, family 3, subfamily A, polypeptide 5
forkhead box O1
1.75 ± 0.17*
heparan sulfate (glucosamine) 3-O-sulfotransferase 1
9.06 ± 1.16*
cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
6.00 ± 1.73*
inhibin, beta B
0.26 ± 0.13*
Substrate-dependent nature of gene upregulation for 3D spheroids
The expressions of special interested genes for MSC spheroids grown on CS and those derived on a non-adherent (polyvinyl alcohol, PVA) substrate were compared by qRT-PCR. The mechanism driving spheroid formation on the non-adherent PVA is similar to that in suspension culture (where a low-attachment dish or flask is used). The self-made PVA substrate, however, is more chemically defined. The results (see Additional file 1: Figure S2) showed significantly higher expression levels of genes including LIF, IL24, TP53, TGF-β3, PDGFRA, and PTGS2 for MSCs grown on CS vs. PVA. The enhanced gene expressions may be attributed to the greater cell-substrate interaction for MSCs on CS through the upregulation of the calcium-associated genes.
Furthermore, the high expression levels for the above-mentioned genes in CS-derived spheroids were maintained or even further enhanced at 72 h as compared with those at 16 h. The sustained regulation of these genes by CS vs. the transient upregulation by PVA suggested the importance of cell-substrate interaction in gene regulation. The differential gene expression in different substrate-derived MSC spheroids also suggested substrate-dependent gene regulation and the critical role of culture substrates in influencing the cell functions and fates even in 3D spheroids.
Discussion and conclusions
The gene expression profiles of CS substrate-derived 3D MSC spheroids vs. 2D cultured MSCs were cross-analyzed by mRNA as well as miRNA microarrays and confirmed by qRT-PCR measurement. The critical role of calcium signaling in CS substrate-derived MSC spheroids was justified by the upregulation of various calcium-associated genes, which has not yet been reported in any other spheroid systems. The unique role of calcium in self-assembled spheroids may be related to the observation that the surface-bound calcium on CS may be translocated into MSCs .
Several kinds of integrin subunit which participate in the processing of cell adhesion or migration were modulated. Besides, members of matrix metalloproteinases (MMPs) were upregulated for MSCs grown on CS. MMPs are proteolytic enzymes that degrade various components of the extracellular matrix (ECM). The proteolytic effects of MMPs play an important role in vascular remodeling, cellular migration, and the processing of ECM proteins and adhesion molecules . The modified chemotactic function of the migrating MSC spheroids was also verified by the upregulation of many chemokines and their receptors. Since the chemotactic function is critical for the therapeutic performance of MSCs, the migration and chemotaxis of MSC spheroids and their link to homing phenomena deserve further investigations.
The multilineage differentiation capacities as well as antiinflammatory and antitumor properties of MSCs may be enhanced after forming spheroids on CS. The antiinflammatory and antitumor properties have been reported in MSC spheroids generated by hanging drop , including the upregulated gene expression of TNFAIP6 and IL24, which were also observed in CS-derived MSC spheroids. The favorable chondrogenic and osteogenic differentiation capacities previously demonstrated for CS-derived MSC spheroids [5, 7] may be associated with the induced expression of TGF-β3 and BMP2 genes. On the other hand, HGF, EGR2, MMP3, and EPHA7 are involved in the development of nervous system [29, 47, 67, 68]. The upregulation of these genes may suggest the enhanced transdifferentiation ability of CS-derived MSC spheroids. Most of all, the upregulation of WNT related genes suggested a profound influence of CS on the fate decision of MSCs. The regulatory changes in the expression of these genes were significantly greater for MSC spheroids derived on CS substrates than those derived on the non-adherent PVA substrates. The critical importance of substrates in stem cell culture, even in the circumstance of 3D spheroid culture, was substantiated in this study. The distinct gene expression profiles on different substrates were in line with the significant substrate-dependent alterations in cell-cell interaction (e.g. cadherins, CAMs, and Notch, etc) and cell-substrate interaction (e.g. integrins) based on the gene analysis.
Finally, it has been mentioned that cells in the core of a 3D spheroid may be exposed to mild hypoxia . The hypoxic environment may resemble the natural niche of MSCs (e.g. O2 tension ~1−7% in bone morrow) more than the normal culture condition (21% O2) . A recent literature has demonstrated that MSCs can benefit from hypoxia to inhibit the senescence, increase the proliferation, and enhance the differentiation potential along the mesenchymal lineages . The hypoxia-inducible factor 1α(HIF-1α) signaling pathway was proposed to be involved in the modulation mechanism of hypoxia effect . Spheroids generated by suspension culture could precondition the human adipose-derived stromal cells  and umbilical vein endothelial cells  to hypoxia environment, leading to upregulations of HIF-1α and angiogenesis. Another recent literature showed that forming 3D spheroids of human gingiva-derived MSCs by suspension culture upregulated many hypoxia-responsive genes, such as HIF-1α, VEGF, SDF-1α, and CXCR4 . The apoptosis signal-regulating kinase 1 (ASK1) and its downstream proteins, the p38α mitogen-activated protein kinase (MAPK) family, act as sensors of oxidative stress [73, 74]. In our study, the upregulation of CXCR4 was observed for MSC spheroids grown on CS. On the other hand, neither the oxidative stress-sensitive genes (including HIF-1α, ASK1, and p38α MAPK) nor the oxidative stress-associated microRNA (such as miR-200a , miR-125b  miR-30b , miR-144  and miR-27b ) were screened out by the mRNA and miRNA microarrays. These results suggested that the oxidative stress and the associated genes may not be activated during the formation of MSC spheroids on CS, and the modulating mechanism of the development-associated genes such as CXCR4 in CS-derived spheroids may be distinct from that in spheroids derived on a non-adherent substrate. This finding reinforces the uniqueness of culture substrates as a microenvironment to predefine the properties of 3D stem cell spheroids.
Although MSCs also formed spheroids on non-adherent substrate (such as PVA), the forming process and the gene regulation profile were not the same as those on CS. On the other hand, MSCs showed similar features of spheroid formation and calcium-related cell behavior on CS substrates even when they were isolated from different tissue sources (adipose, placenta, or umbilical cord) or species (human or rat) [5, 9]. The capacity of MSCs to form spheroids on CS was more influenced by their stemness , and the surface-bound calcium on the substrate . In this study, we observed that a few genes were regulated during spheroid formation which may participate in calcium signaling pathway. However, the critical genes that turn on the mechanism as well as the link between calcium regulation and the genes involved in different cell functions (adhesion, migration, antiinflammatory, and differentiation) remains unknown. These issues are interesting and worthy of further investigations. The gene regulation profile screened by the cross-correlation analysis described here may provide helpful information for studying these unique substrate-induced MSC spheroids.
Isolation and culture of MSCs
All human subjects and protocols involved were approved by the institutional review board of Chang Gung Memorial Hospital (IRB#92-176). The fresh umbilical cords were collected at the hospital after obtaining written informed consent from each donor participating in this study. The informed consent is always obtained from the mother. The blood vessels were removed by washing with PBS. The cleaned-up tissue was sliced into small pieces and digested with 0.05% trypsin and 300 U/ml collagenase in alpha minimum essential medium (α-MEM, Gibco) for 1 h at 37°C. Cells were gathered from pellets after centrifugation and incubated at 37°C with 5% CO2. The culture medium consisted of α-MEM supplemented with 10% fetal bovine serum (Hyclone), 10 mg/l penicillin-streptomycin, and 10 mg/l l-glutamine (Gibco). On the next day, non-adherent cells were removed. The medium was refreshed two times every week. Cells of the 2nd to the 6th passages were used in this study.
Analysis of surface markers for the human MSCs
Surface markers for human umbilical cord MSCs were quantified by flow cytometry using CD13, CD14, CD29, CD31, CD34, CD44, CD45, CD56, CD59, CD61, CD71, CD105, CD106, CD133, HLA-ABC, HLA-DR (all from BioLegend), CD73 (BD Pharmingen), and CD90 (Serotec) antibodies. MSCs (5×105 cells) were washed twice with PBS, resuspended in 100 μl of PBS containing monoclonal antibodies, and incubated for 30 min at 4°C. These cells were then washed twice and resuspended in 500 μl of PBS. Fluorescence analysis was performed with a flow cytometer (FACS Caliber, BD). The non-specific binding of the fluorescein isothiocyanate (FITC) and phosphatidyl ethanolamine (PE) conjugates were determined in control samples using a mouse IgG1-FITC and IgG1-PE negative control (Serotec). Analysis was conducted using the WinMDI 2.9 software.
Preparation of chitosan (CS) membranes as the culture substrate
CS powder (molecular weight ~416 kDa, 77.7% deacetylation, Fluka) was dissolved and stirred in 1% aqueous acetic acid solution for 24 h at room temperature to obtain a 1% CS solution. The 1% CS solution was casted on 6-well tissue culture plate (1.5 ml/ well) or 15 mm microscope coverslip glass (300 μl/ slip) and air-dried for 2 days. The CS substrates were treated with 0.5 N NaOH in 75% ethanol for 5 minutes, and then washed extensively by distilled water. These CS substrates were further antiseptically rinsed with 75% ethanol and washed by phosphate buffer saline (PBS) before use. The static water contact angle of CS membranes were determined by a contact angle meter (FTA, USA). The surface zeta potential was determined by electrophoretic light scattering using the Delsa Nano C Analyzer (Beckman Coulter, USA) with a flat solid cell. To analysis the amount of surface-bound calcium on substrates, CS-coated coverslip glass was placed into the well of a 24-well tissue culture plate where 1 ml of culture medium was added. After incubation at 37°C for 24 h or 72 h, the medium was collected for later analysis of the free calcium ion remained in the bulk solution. A blank well (TCPS) was used as the control. The concentration of calcium in each of the collected solution was measured by the atomic absorption (AA) spectrometry (iCE 3300; Thermo Scientific, USA). The content of surface-bound calcium was calculated by subtracting the amount of calcium remained in the collected solution from those in stock culture medium.
MSC culture on CS membranes
MSCs (2.5 × 105 cells per well for 6-well plate) were seeded in each well and the morphology of cells on the membranes was observed by an inverted microscope (Leica DMIRB). Cells seeded in the culture well (tissue culture polystyrene, TCPS) served as the control. The average diameters of spheroids were quantified from the images. The cell viability in MSC spheroids was determined by using propidium iodide (PI) (Sigma) staining and flow cytometry. After grown on CS for 24 h, MSC spheroids were collected and dissociated in 0.25% trypsin-EDTA solution for 10 min at room temperature. These cells were then washed and resuspended in 500 μl of PBS. The solution of PI (concentration 2 mg/ml) was added to cell suspension before the analysis by the flow cytometer. The percentage of cells without being stained by PI was defined as the cell viability.
Analysis of gene and miRNA expression microarray
To understand the signaling events involved in the spheroid formation on CS, MSCs of the 6th passage were cultured on TCPS or CS substrates for 16 h. Total RNA of these MSCs were extracted, and then analyzed by gene and miRNA expression microarrays. As for the analysis of gene expression, treatment RNA (CS substrates) was labeled by Cy5 and RNA from human reference RNA (TCPS) was labeled by Cy3. Cy-labeled cRNA (2 μg) was fragmented to an average size of about 50-100 nucleotides by incubation with fragmentation buffer at 60°C for 30 min. Correspondingly, fragmented-labeled cRNA was then pooled and hybridized to the Human 1A (version 2) gene expression microarray (Agilent Technologies) at 60°C for 17 h. After washing and drying in nitrogen, the microarrays were scanned with the Agilent microarray scanner at 535 nm for Cy3 and at 625 nm for Cy5. Scanned images were analyzed using Feature Extraction software 10.7 (Agilent Technologies). Only the microarray images with signal significant ratios > 3 in either the Cy3 or Cy5 channel were retrieved for further analysis.
On the other hand, the miRNA was isolated by using miRNeasy Mini kits (Qiagen) followed by quality checks of both total RNA and small RNA using a 2100 Bioanalyzer and software which detected 28S and 18S ribosomal RNA ratios, generated a RNA Integrity Number (RIN), concentration of sample, and ribosomal ratio. Only samples with 28S/18S > 1.2, RIN > 8, and detectable miRNA were used for this study. The Agilent customer Human R16 miRNA array was employed for this study following manufacturer's protocols. The screened data of miRNA microarray were analyzed by the software, GeneSpring 7.3.1 (Agilent Technologies). The miRNA microarray images with signal ratio greater or lower than three times were screened out and defined as the normalized miRNA profile for further analysis.
qRT-PCR confirmation for the genes screened by cross-correlation analysis of microarrays
In order to further validate the results derived from microarrays, qRT-PCR was performed for special interested genes. In brief, total RNA of cells at the end of the culture period was extracted by the Trizol® reagent (Invitrogen) according to the manufacturer’s instructions. Human MSCs cDNA synthesis and amplification via qRT-PCR were performed using the RevertAidTM First Strand cDNA Synthesis Kit (Thermo, Fermentas). Paired forward and reverse primers were designed from UniSTS database in National Center for Biotechnology Information. The 100 ng of cDNA was used for quantitative real-time PCR using the GM SYBR qPCR Kits (GeneMark, Taiwan) with 150 nM targeted gene oligonucleotide primer pairs. 40 cycles of PCR consisting of denaturing at 95°C for 2 s (3 min in the first cycle), annealing and extension for 30 s were performed by a Chrom4 Thermal Cycler System (MJ Research). The value of each sample was normalized to the expression of the GAPDH housekeeping gene in the same sample. The primer sequences for each gene used in this study are shown in Additional file 4: Table S1).
Multiple samples were used in each experiment. Numerical values were expressed as the mean ± standard deviation. Statistical differences among the experimental groups were evaluated by two-tailed student’s t-test. A significant difference was considered when P ≤ 0.05. In all studies, three independent experiments were performed for each type of experiments.
This work was supported by the Program for Stem Cell and Regenerative Medicine Frontier Research (NSC101-2321-B-002-039) sponsored by the National Science Council, Taiwan, R.O.C.
- Gerecht-Nir S, Cohen S, Itskovitz-Eldor J: Bioreactor cultivation enhances the efficiency of human embryoid body (hEB) formation and differentiation. Biotechnol Bioeng. 2004, 86 (5): 493-502. 10.1002/bit.20045.PubMedView ArticleGoogle Scholar
- Bartosh TJ, Ylöstalo JH, Mohammadipoor A, Bazhanov N, Coble K, Claypool K, Lee RH, Choi H, Prockop DJ: Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties. Proc Natl Acad Sci U S A. 2010, 107 (31): 13724-13729. 10.1073/pnas.1008117107.PubMed CentralPubMedView ArticleGoogle Scholar
- Frith JE, Thomson B, Genever PG: Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential. Tissue Eng Part C Methods. 2010, 16 (4): 735-749. 10.1089/ten.tec.2009.0432.PubMedView ArticleGoogle Scholar
- Wang W, Itaka K, Ohba S, Nishiyama N, Chung UI, Yamasaki Y, Kataoka K: 3D spheroid culture system on micropatterned substrates for improved differentiation efficiency of multipotent mesenchymal stem cells. Biomaterials. 2009, 30 (14): 2705-2715. 10.1016/j.biomaterials.2009.01.030.PubMedView ArticleGoogle Scholar
- Huang GS, Dai LG, Yen BL, Hsu SH: Spheroid formation of mesenchymal stem cells on chitosan and chitosan-hyaluronan membranes. Biomaterials. 2011, 32 (29): 6929-6945. 10.1016/j.biomaterials.2011.05.092.PubMedView ArticleGoogle Scholar
- Cheng NC, Wang S, Young TH: The influence of spheroid formation of human adipose-derived stem cells on chitosan films on stemness and differentiation capabilities. Biomaterials. 2012, 33 (6): 1748-1758. 10.1016/j.biomaterials.2011.11.049.PubMedView ArticleGoogle Scholar
- Mathews S, Gupta PK, Bhonde R, Totey S: Chitosan enhances mineralization during osteoblast differentiation of human bone marrow-derived mesenchymal stem cells, by upregulating the associated genes. Cell Prolif. 2011, 44 (6): 537-549. 10.1111/j.1365-2184.2011.00788.x.PubMedView ArticleGoogle Scholar
- Hsu SH, Huang GS, Feng F: Isolation of the multipotent MSC subpopulation from human gingival fibroblasts by culturing on chitosan membranes. Biomaterials. 2012, 33 (9): 2642-2655. 10.1016/j.biomaterials.2011.12.032.PubMedView ArticleGoogle Scholar
- Yeh H-Y, Liu B-H, Hsu S-h: The calcium-dependent regulation of spheroid formation and cardiomyogenic differentiation for MSCs on chitosan membranes. Biomaterials. 2012, 33 (35): 8943-8954. 10.1016/j.biomaterials.2012.08.069.PubMedView ArticleGoogle Scholar
- Dalby MJ, Andar A, Nag A, Affrossman S, Tare R, McFarlane S, Oreffo ROC: Genomic expression of mesenchymal stem cells to altered nanoscale topographies. J R Soc Interface. 2008, 5 (26): 1055-1065. 10.1098/rsif.2008.0016.PubMed CentralPubMedView ArticleGoogle Scholar
- Tripathi R, Saini HK, Rad R, Abreu-Goodger C, van Dongen S, Enright AJ: Messenger RNA and microRNA profiling during early mouse EB formation. Gene Expr Patterns. 2011, 11 (5–6): 334-344.PubMedView ArticleGoogle Scholar
- Bosch J, Houben AP, Radke TF, Stapelkamp D, Bunemann E, Balan P, Buchheiser A, Liedtke S, Kogler G: Distinct differentiation potential of "MSC" derived from cord blood and umbilical cord: are cord-derived cells true mesenchymal stromal cells?. Stem Cells Dev. 2012, 21 (11): 1977-1988. 10.1089/scd.2011.0414.PubMedView ArticleGoogle Scholar
- Lu LL, Liu YJ, Yang SG, Zhao QJ, Wang X, Gong W, Han ZB, Xu ZS, Lu YX, Liu D, et al: Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials. Haematologica. 2006, 91 (8): 1017-1026.PubMedGoogle Scholar
- Yeh HY, Lin JC: Surface phosphorylation for polyelectrolyte complex of chitosan and its sulfonated derivative: surface analysis, blood compatibility and adipose derived stem cell contact properties. J Biomater Sci Polym Ed. 2012, 23 (1–4): 233-250.PubMedView ArticleGoogle Scholar
- Li B, Wang Y, Jia D, Zhou Y: Gradient structural bone-like apatite induced by chitosan hydrogel via ion assembly. J Biomater Sci Polym Ed. 2011, 22 (4–6): 505-517.PubMedView ArticleGoogle Scholar
- Okunade GW, Miller ML, Pyne GJ, Sutliff RL, O'Connor KT, Neumann JC, Andringa A, Miller DA, Prasad V, Doetschman T, et al: Targeted ablation of plasma membrane Ca2+-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4. J Biol Chem. 2004, 279 (32): 33742-33750. 10.1074/jbc.M404628200.PubMedView ArticleGoogle Scholar
- Akaike A: Functional changes induced by long-term stimulation of nicotinic acetylcholine receptors. Foreword. Biol Pharm Bul. 2009, 32 (3): 317-10.1248/bpb.32.317.View ArticleGoogle Scholar
- Weick JP, Austin Johnson M, Zhang SC: Developmental regulation of human embryonic stem cell-derived neurons by calcium entry via transient receptor potential channels. Stem Cells. 2009, 27 (12): 2906-2916.PubMed CentralPubMedGoogle Scholar
- Bayer H, Muller T, Myrtek D, Sorichter S, Ziegenhagen M, Norgauer J, Zissel G, Idzko M: Serotoninergic receptors on human airway epithelial cells. Am J Respir Cell Mol Biol. 2007, 36 (1): 85-93. 10.1165/rcmb.2006-0151OC.PubMedView ArticleGoogle Scholar
- Ichimonji I, Tomura H, Mogi C, Sato K, Aoki H, Hisada T, Dobashi K, Ishizuka T, Mori M, Okajima F: Extracellular acidification stimulates IL-6 production and Ca(2+) mobilization through proton-sensing OGR1 receptors in human airway smooth muscle cells. Am J Physiol Lung Cell Mol Physiol. 2010, 299 (4): L567-577. 10.1152/ajplung.00415.2009.PubMedView ArticleGoogle Scholar
- Isa M, Ohta Y, Namiki S, Hirose K: Artificial control of subtype-specific platelet-derived growth factor-receptor signaling. J Pharmacol Sci. 2009, 111 (3): 312-316. 10.1254/jphs.09136SC.PubMedView ArticleGoogle Scholar
- Grade S, Agasse F, Bernardino L, Silva CG, Cortes L, Malva JO: Functional identification of neural stem cell-derived oligodendrocytes by means of calcium transients elicited by thrombin. Rejuvenation Res. 2010, 13 (1): 27-37. 10.1089/rej.2009.0889.PubMedView ArticleGoogle Scholar
- Hatziapostolou M, Koukos G, Polytarchou C, Kottakis F, Serebrennikova O, Kuliopulos A, Tsichlis PN: Tumor progression locus 2 mediates signal-induced increases in cytoplasmic calcium and cell migration. Sci Signal. 2011, 4 (187): ra55-PubMed CentralPubMedView ArticleGoogle Scholar
- Parys JB, De Smedt H: Inositol 1,4,5-trisphosphate and its receptors. Adv Exp Med Biol. 2012, 740: 255-279. 10.1007/978-94-007-2888-2_11.PubMedView ArticleGoogle Scholar
- Ozaki N, Miura Y, Yamada T, Kato Y, Oiso Y: RasGRP3 mediates phorbol ester-induced, protein kinase C-independent exocytosis. Biochem Biophys Res Commun. 2005, 329 (2): 765-771. 10.1016/j.bbrc.2005.02.031.PubMedView ArticleGoogle Scholar
- Gentile MT, Reccia MG, Sorrentino PP, Vitale E, Sorrentino G, Puca AA, Colucci-D'Amato L: Role of cytosolic calcium-dependent phospholipase A2 in Alzheimer's disease pathogenesis. Mol Neurobiol. 2012, 45 (3): 596-604. 10.1007/s12035-012-8279-4.PubMedView ArticleGoogle Scholar
- Yanez M, Gil-Longo J, Campos-Toimil M: Calcium binding proteins. Adv Exp Med Biol. 2012, 740: 461-482. 10.1007/978-94-007-2888-2_19.PubMedView ArticleGoogle Scholar
- Ho IA, Chan KY, Ng WH, Guo CM, Hui KM, Cheang P, Lam PY: Matrix metalloproteinase 1 is necessary for the migration of human bone marrow-derived mesenchymal stem cells toward human glioma. Stem Cells. 2009, 27 (6): 1366-1375. 10.1002/stem.50.PubMed CentralPubMedView ArticleGoogle Scholar
- Van Hove I, Lemmens K, Van de Velde S, Verslegers M, Moons L: Matrix metalloproteinase-3 in the central nervous system: a look on the bright side. J Neurochem. 2012, 123 (2): 203-216. 10.1111/j.1471-4159.2012.07900.x.PubMedView ArticleGoogle Scholar
- Shibata T, Shimoyama Y, Gotoh M, Hirohashi S: Identification of human cadherin-14, a novel neurally specific type II cadherin, by protein interaction cloning. J Biol Chem. 1997, 272 (8): 5236-5240. 10.1074/jbc.272.8.5236.PubMedView ArticleGoogle Scholar
- Aamar E, Dawid IB: Protocadherin-18a has a role in cell adhesion, behavior and migration in zebrafish development. Developmental Biology. 2008, 318 (2): 335-346. 10.1016/j.ydbio.2008.03.040.PubMedView ArticleGoogle Scholar
- Li L, Bennett SA, Wang L: Role of E-cadherin and other cell adhesion molecules in survival and differentiation of human pluripotent stem cells. Cell Adh Migr. 2012, 6 (1): 59-70. 10.4161/cam.19583.PubMed CentralPubMedView ArticleGoogle Scholar
- Perdigoto CN, Bardin AJ: Sending the right signal: Notch and stem cells. Biochim Biophys Acta 2012.Google Scholar
- Pasquale EB: Eph-ephrin bidirectional signaling in physiology and disease. Cell. 2008, 133 (1): 38-52. 10.1016/j.cell.2008.03.011.PubMedView ArticleGoogle Scholar
- Pietri S, Dimidschstein J, Tiberi L, Sotiropoulou PA, Bilheu A, Goffinet A, Achouri Y, Tissir F, Blanpain C, Jacquemin P, et al: Transcriptional mechanisms of EphA7 gene expression in the developing cerebral cortex. Cereb Cortex. 2012, 22 (7): 1678-1689. 10.1093/cercor/bhr245.PubMedView ArticleGoogle Scholar
- Yoshimura T, Oppenheim JJ: Chemokine-like receptor 1 (CMKLR1) and chemokine (C-C motif) receptor-like 2 (CCRL2); two multifunctional receptors with unusual properties. Exp Cell Res. 2011, 317 (5): 674-684. 10.1016/j.yexcr.2010.10.023.PubMed CentralPubMedView ArticleGoogle Scholar
- Singh AK, Arya RK, Trivedi AK, Sanyal S, Baral R, Dormond O, Briscoe DM, Datta D: Chemokine receptor trio: CXCR3, CXCR4 and CXCR7 crosstalk via CXCL11 and CXCL12. Cytokine Growth Factor Rev 2012.Google Scholar
- Xu F, Shi J, Yu B, Ni W, Wu X, Gu Z: Chemokines mediate mesenchymal stem cell migration toward gliomas in vitro. Oncol Rep. 2010, 23 (6): 1561-1567.PubMedGoogle Scholar
- Schenk S, Mal N, Finan A, Zhang M, Kiedrowski M, Popovic Z, McCarthy PM, Penn MS: Monocyte chemotactic protein-3 is a myocardial mesenchymal stem cell homing factor. Stem Cells. 2007, 25 (1): 245-251. 10.1634/stemcells.2006-0293.PubMedView ArticleGoogle Scholar
- Goss AM, Tian Y, Cheng L, Yang J, Zhou D, Cohen ED, Morrisey EE: Wnt2 signaling is necessary and sufficient to activate the airway smooth muscle program in the lung by regulating myocardin/Mrtf-B and Fgf10 expression. Developmental biology. 2011, 356 (2): 541-552. 10.1016/j.ydbio.2011.06.011.PubMed CentralPubMedView ArticleGoogle Scholar
- Onizuka T, Yuasa S, Kusumoto D, Shimoji K, Egashira T, Ohno Y, Kageyama T, Tanaka T, Hattori F, Fujita J, et al: Wnt2 accelerates cardiac myocyte differentiation from ES-cell derived mesodermal cells via non-canonical pathway. J Mol Cell Cardiol. 2012, 52 (3): 650-659. 10.1016/j.yjmcc.2011.11.010.PubMedView ArticleGoogle Scholar
- Port F, Basler K: Wnt trafficking: new insights into Wnt maturation, secretion and spreading. Traffic. 2010, 11 (10): 1265-1271. 10.1111/j.1600-0854.2010.01076.x.PubMedView ArticleGoogle Scholar
- Mao CD, Byers SW: Cell-context dependent TCF/LEF expression and function: alternative tales of repression, de-repression and activation potentials. Crit Rev Eukaryot Gene Expr. 2011, 21 (3): 207-236. 10.1615/CritRevEukarGeneExpr.v21.i3.10.PubMed CentralPubMedView ArticleGoogle Scholar
- Li D, Hallett MA, Zhu W, Rubart M, Liu Y, Yang Z, Chen H, Haneline LS, Chan RJ, Schwartz RJ, et al: Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis. Development. 2011, 138 (2): 303-315. 10.1242/dev.055566.PubMed CentralPubMedView ArticleGoogle Scholar
- Kojima T, Shimazui T, Hinotsu S, Joraku A, Oikawa T, Kawai K, Horie R, Suzuki H, Nagashima R, Yoshikawa K, et al: Decreased expression of CXXC4 promotes a malignant phenotype in renal cell carcinoma by activating Wnt signaling. Oncogene. 2009, 28 (2): 297-305. 10.1038/onc.2008.391.PubMedView ArticleGoogle Scholar
- Rhinn M, Dolle P: Retinoic acid signalling during development. Development. 2012, 139 (5): 843-858. 10.1242/dev.065938.PubMedView ArticleGoogle Scholar
- Li S, Miao T, Sebastian M, Bhullar P, Ghaffari E, Liu M, Symonds AL, Wang P: The transcription factors Egr2 and Egr3 are essential for the control of inflammation and antigen-induced proliferation of B and T cells. Immunity. 2012, 37 (4): 685-696. 10.1016/j.immuni.2012.08.001.PubMed CentralPubMedView ArticleGoogle Scholar
- Yin P, Navarro A, Fang F, Xie A, Coon JS, Richardson C, Bulun SE: Early growth response-2 expression in uterine leiomyoma cells: regulation and function. Fertil Steril. 2011, 96 (2): 439-444. 10.1016/j.fertnstert.2011.05.062.PubMed CentralPubMedView ArticleGoogle Scholar
- Milovanovic M, Volarevic V, Radosavljevic G, Jovanovic I, Pejnovic N, Arsenijevic N, Lukic ML: IL-33/ST2 axis in inflammation and immunopathology. Immunol Res. 2012, 52 (1–2): 89-99.PubMedView ArticleGoogle Scholar
- Davidson A: Targeting BAFF in autoimmunity. Curr Opin Immunol. 2010, 22 (6): 732-739. 10.1016/j.coi.2010.09.010.PubMed CentralPubMedView ArticleGoogle Scholar
- Volarevic V, Al-Qahtani A, Arsenijevic N, Pajovic S, Lukic ML: Interleukin-1 receptor antagonist (IL-1Ra) and IL-1Ra producing mesenchymal stem cells as modulators of diabetogenesis. Autoimmunity. 2010, 43 (4): 255-263. 10.3109/08916930903305641.PubMedView ArticleGoogle Scholar
- Carbonnelle-Puscian A, Copie-Bergman C, Baia M, Martin-Garcia N, Allory Y, Haioun C, Cremades A, Abd-Alsamad I, Farcet JP, Gaulard P, et al: The novel immunosuppressive enzyme IL4I1 is expressed by neoplastic cells of several B-cell lymphomas and by tumor-associated macrophages. Leukemia. 2009, 23 (5): 952-960. 10.1038/leu.2008.380.PubMed CentralPubMedView ArticleGoogle Scholar
- Nasef A, Mazurier C, Bouchet S, Francois S, Chapel A, Thierry D, Gorin NC, Fouillard L: Leukemia inhibitory factor: role in human mesenchymal stem cells mediated immunosuppression. Cell Immunol. 2008, 253 (1–2): 16-22.PubMedView ArticleGoogle Scholar
- Fujio Y, Maeda M, Mohri T, Obana M, Iwakura T, Hayama A, Yamashita T, Nakayama H, Azuma J: Glycoprotein 130 cytokine signal as a therapeutic target against cardiovascular diseases. J Pharmacol Sci. 2011, 117 (4): 213-222. 10.1254/jphs.11R05CR.PubMedView ArticleGoogle Scholar
- Prockop DJ, Oh JY: Mesenchymal stem/stromal cells (MSCs): role as guardians of inflammation. Mol Ther. 2012, 20 (1): 14-20. 10.1038/mt.2011.211.PubMed CentralPubMedView ArticleGoogle Scholar
- Kwon B: Regulation of Inflammation by Bidirectional Signaling through CD137 and Its Ligand. Immune Netw. 2012, 12 (5): 176-180. 10.4110/in.2012.12.5.176.PubMed CentralPubMedView ArticleGoogle Scholar
- Rizzo MT: Cyclooxygenase-2 in oncogenesis. Clin Chim Acta. 2011, 412 (9–10): 671-687.PubMedView ArticleGoogle Scholar
- Soleymaninejadian E, Pramanik K, Samadian E: Immunomodulatory properties of mesenchymal stem cells: cytokines and factors. Am J Reprod Immunol. 2012, 67 (1): 1-8. 10.1111/j.1600-0897.2011.01069.x.PubMedView ArticleGoogle Scholar
- Tamai H, Miyake K, Yamaguchi H, Takatori M, Dan K, Inokuchi K, Shimada T: AAV8 vector expressing IL24 efficiently suppresses tumor growth mediated by specific mechanisms in MLL/AF4-positive ALL model mice. Blood. 2012, 119 (1): 64-71. 10.1182/blood-2011-05-354050.PubMedView ArticleGoogle Scholar
- Zhu W, Wei L, Zhang H, Chen J, Qin X: Oncolytic adenovirus armed with IL-24 Inhibits the growth of breast cancer in vitro and in vivo. J Exp Clin Cancer Res. 2012, 31: 51-10.1186/1756-9966-31-51.PubMed CentralPubMedView ArticleGoogle Scholar
- Nii T, Marumoto T, Tani K: Roles of p53 in various biological aspects of hematopoietic stem cells. J Biomed Biotechnol. 2012, 2012: 903435-PubMed CentralPubMedView ArticleGoogle Scholar
- Lou Y, Liu S: The TIPE (TNFAIP8) family in inflammation, immunity, and cancer. Mol Immunol. 2011, 49 (1–2): 4-7.PubMedView ArticleGoogle Scholar
- Barouki R, Aggerbeck M, Aggerbeck L, Coumoul X: The aryl hydrocarbon receptor system. Drug Metabol Drug Interact. 2012, 27 (1): 3-8.PubMedView ArticleGoogle Scholar
- Singh AK, Kashyap MP, Jahan S, Kumar V, Tripathi VK, Siddiqui MA, Yadav S, Khanna VK, Das V, Jain SK, et al: Expression and inducibility of cytochrome P450s (CYP1A1, 2B6, 2E1, 3A4) in human cord blood CD34(+) stem cell-derived differentiating neuronal cells. Toxicol Sci. 2012, 129 (2): 392-410. 10.1093/toxsci/kfs213.PubMedView ArticleGoogle Scholar
- Chiang TS, Yang KC, Zheng SK, Chiou LL, Hsu WM, Lin FH, Huang GT, Lee HS: The prediction of drug metabolism using scaffold-mediated enhancement of the induced cytochrome P450 activities in fibroblasts by hepatic transcriptional regulators. Biomaterials. 2012, 33 (21): 5187-5197. 10.1016/j.biomaterials.2012.04.014.PubMedView ArticleGoogle Scholar
- Raffetto JD, Khalil RA: Matrix metalloproteinases and their inhibitors in vascular remodeling and vascular disease. Biochem Pharmacol. 2008, 75 (2): 346-359. 10.1016/j.bcp.2007.07.004.PubMed CentralPubMedView ArticleGoogle Scholar
- Zheng B, Wang C, He L, Xu X, Qu J, Hu J, Zhang H: Neural differentiation of mesenchymal stem cells influences chemotactic responses to HGF. J Cell Physiol. 2013, 228 (1): 149-162. 10.1002/jcp.24114.PubMedView ArticleGoogle Scholar
- Mellott DO, Burke RD: Divergent roles for Eph and ephrin in avian cranial neural crest. BMC Dev Biol. 2008, 8: 56-10.1186/1471-213X-8-56.PubMed CentralPubMedView ArticleGoogle Scholar
- Bhang SH, Cho SW, La WG, Lee TJ, Yang HS, Sun AY, Baek SH, Rhie JW, Kim BS: Angiogenesis in ischemic tissue produced by spheroid grafting of human adipose-derived stromal cells. Biomaterials. 2011, 32 (11): 2734-2747. 10.1016/j.biomaterials.2010.12.035.PubMedView ArticleGoogle Scholar
- Tsai CC, Yew TL, Yang DC, Huang WH, Hung SC: Benefits of hypoxic culture on bone marrow multipotent stromal cells. Am J Blood Res. 2012, 2 (3): 148-159.PubMed CentralPubMedGoogle Scholar
- Bhang SH, Lee S, Lee TJ, La WG, Yang HS, Cho SW, Kim BS: Three-dimensional cell grafting enhances the angiogenic efficacy of human umbilical vein endothelial cells. Tissue Eng Part A. 2012, 18 (3–4): 310-319.PubMed CentralPubMedView ArticleGoogle Scholar
- Zhang Q, Nguyen AL, Shi S, Hill C, Wilder-Smith P, Krasieva TB, Le AD: Three-dimensional spheroid culture of human gingiva-derived mesenchymal stem cells enhances mitigation of chemotherapy-induced oral mucositis. Stem Cells Dev. 2012, 21 (6): 937-947. 10.1089/scd.2011.0252.PubMed CentralPubMedView ArticleGoogle Scholar
- Soga M, Matsuzawa A, Ichijo H: Oxidative Stress-Induced Diseases via the ASK1 Signaling Pathway. Int J Cell Biol. 2012, 2012: 439587-PubMed CentralPubMedView ArticleGoogle Scholar
- Mateescu B, Batista L, Cardon M, Gruosso T, de Feraudy Y, Mariani O, Nicolas A, Meyniel JP, Cottu P, Sastre-Garau X, et al: miR-141 and miR-200a act on ovarian tumorigenesis by controlling oxidative stress response. Nat Med. 2011, 17 (12): 1627-1635. 10.1038/nm.2512.PubMedView ArticleGoogle Scholar
- Manca S, Magrelli A, Cialfi S, Lefort K, Ambra R, Alimandi M, Biolcati G, Uccelletti D, Palleschi C, Screpanti I, et al: Oxidative stress activation of miR-125b is part of the molecular switch for Hailey-Hailey disease manifestation. Exp Dermatol. 2011, 20 (11): 932-937. 10.1111/j.1600-0625.2011.01359.x.PubMedView ArticleGoogle Scholar
- Haque R, Chun E, Howell JC, Sengupta T, Chen D, Kim H: MicroRNA-30b-mediated regulation of catalase expression in human ARPE-19 cells. PLoS ONE. 2012, 7 (8): e42542-10.1371/journal.pone.0042542.PubMed CentralPubMedView ArticleGoogle Scholar
- Sangokoya C, Telen MJ, Chi JT: microRNA miR-144 modulates oxidative stress tolerance and associates with anemia severity in sickle cell disease. Blood. 2010, 116 (20): 4338-4348. 10.1182/blood-2009-04-214817.PubMed CentralPubMedView ArticleGoogle Scholar
- Thulasingam S, Massilamany C, Gangaplara A, Dai H, Yarbaeva S, Subramaniam S, Riethoven JJ, Eudy J, Lou M, Reddy J: miR-27b*, an oxidative stress-responsive microRNA modulates nuclear factor-kB pathway in RAW 264.7 cells. Mol Cell Biochem. 2011, 352 (1-2): 181-188. 10.1007/s11010-011-0752-2.PubMedView ArticleGoogle Scholar
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