Figure 2

Flow cytometric analysis of genetically complemented strains. (A) The chromosome equivalent profiles of the complementation strains ∆ctrA pHW1 (red line), ∆cckA pHW2 (green line) and ∆chpT pHW3 (blue line) are compared to those of the respective knock-out mutants (black line) and the wild-type (grey area). The analysis was carried out with cultures in the mid-exponential phase. (B) Differential expression of ctrA (red), cckA (green) and chpT (blue) in the complemented strains (pHW1-3) in trans compared to the wild-type strain was tested by RT-qPCR. The results of two biological replicates are shown.