Table 3 Oligonucleotide primers used for qPCR designed against GenBank sequence available for microarray features
From: Gene duplication in an African cichlid adaptive radiation
GenBank | Primer sequence | Homology | Primer efficiency | ||
|---|---|---|---|---|---|
Predicted length | A. burtoni | Test species | |||
DY626766 | F: TCGGTCTCCTTAACCGGATG | No Hit | 193 | 86 | 74 |
R: CTGAGTTTGGCTGCCCGTAA | (P. similis) | ||||
DY627986 | F: ACGAACACCCGAACGGAAAC | Hox gene cluster | 222 | 100 | 104 |
R: GGTGCACGCACATGAACTGT | (M. estherae) | ||||
DY631898 | F: CGTCCCAGTGAGGATGAGGA | MHC class II antigen | 161 | 82 | 82 |
R: TGATGCTGATCGGTTGATGC | (R. “chilingali”) | ||||
DY632057 | F: ATTACTGCGAGTGCCGTCCA | Pituitary adenylate cyclase activating | 150 | 91 | 78 |
R: CTGCGCCCTGAAAGAACAGA | polypeptide receptor 1A | (A. tweddlei) | |||