Figure 4

Distribution of RAD paired-end contig lengths. Paired-end (PE) RAD-sequencing generates a read from the P1 adaptor which is located at the restriction site and a PE contig generated from sequencing at the P2 adaptor at the sheared end of the fragment. The most frequent length of the PE contigs was between 450 and 600 bp. A small number of PE contigs were over 650 bp in length; these are not shown in the figure.