Figure 1

Sequencing of C. elegans sperm transcriptome. (a) Microscopic image of purified sperm cells. (b) Whole worms and concentrated sperm were analyzed by immunoblotting using antibodies against mono-methylated H3K4 and tubulin. No tubulin was detected in the sperm suggesting high purity of the prepared sample. (c)  Classification of the sperm transcriptome sequencing reads. Most of the reads are coding sequences (CDS); about ~10% of the reads are non-coding sequences including those with predicted coding potential. Reads shorter than 50 bp, unmapped to the C. elegans genome or coding tRNAs, rRNAs, snoRNAs and snRNAs were filtered out before assembly.