Transcriptome analysis identifies Bacillus anthracis genes that respond to CO2through an AtxA-dependent mechanism

Table 4 Correlation of qPCR expression with RNA-sequencing expression for selected genes

Locus tag	Name and/or function of product	Pearson’s R	P-Value
sRNA-1	Unknown	0.99	< 0.0001
GBAA_pXO1_0124	bslA, adhesin	0.99	< 0.0001
sRNA-2	Unknown	0.99	< 0.0001
GBAA0887	Eag, surface layer protein	0.89	0.0077
GBAA1639	GerN, germination protein	0.99	< 0.0001
GBAA2840	Transporter, putative	0.99	< 0.0001
GBAA4875	Universal stress protein family	0.86	0.0127

For qPCR, gene expression was normalized to the expression of the housekeeping gene, GBAA3936, encoding aspartokinase. Sample Pearson correlation coefficients were calculated for each gene in all eight samples.

ISSN: 1471-2164