Table 2 Efficiency variables for individual RT-qPCR genes
Gene | Optimum [primer] μM | Slope (−) | R2 | Efficiency |
|---|---|---|---|---|
ACTA2 | 5 | 3.62 | 0.99 | 1.890 |
APEH | 20 | 3.18 | 0.99 | 2.064 |
CELA1 | 10 | 3.54 | 0.99 | 1.917 |
DONSON | 10 | 3.20 | 0.99 | 2.054 |
FST | 2.5 | 3.60 | 0.97 | 1.896 |
GALNT6 | 5 | 3.29 | 0.99 | 2.103 |
GJA1 | 20 | 3.31 | 0.98 | 2.003 |
IL33 | 5 | 3.33 | 0.96 | 1.997 |
MMP19 | 10 | 3.52 | 0.99 | 1.925 |
MOSC2 | 10 | 3.14 | 0.99 | 2.082 |
NMB | 10 | 3.21 | 0.99 | 2.049 |
NPPC | 5 | 3.63 | 0.99 | 1.885 |
PPARA | 5 | 3.33 | 0.99 | 1.997 |
RAB3B | 20 | 3.51 | 0.99 | 1.927 |
SFRP1 | 5 | 3.70 | 0.97 | 1.863 |
SLC1A3 | 10 | 3.46 | 0.97 | 1.946 |
SLC45A2 | 10 | 3.54 | 0.95 | 1.916 |
TGFB1I1 | 20 | 3.29 | 0.96 | 2.013 |
UBQ | 2.5 | 3.20 | 0.99 | 2.054 |