Figure 3

The relative level of expression of eleven caleosin paralogs measured in a panel of triticale tissues. The expression of thirty-two caleosin gene family members was individually measured in 454 cDNA sequence libraries from anther, pollen, root, stem, and stigma triticale tissues using RNA-seq analysis. The aligned 454-cDNAs to each caleosin member were counted, then normalized based on gene lengths and library depths using the RPKM method. The expression of each paralog is subdivided into expression of each of the three homeologs, visualized as black bars for the R subgenome, grey bars for the B subgenome, and white bars for the A subgenome. Clo2 represented the expression of only the A and B homeologs. Values are the total RPKM of two replicates for stigma and pollen, two replicates each for UNM (uninucleate microspore) and TCP (tricellular pollen) anther tissues, and six replicates for stem. A one-way ANOVA was carried out to test the significance of global differences in caleosin gene expression in each triticale tissue. Duncan’s multiple range test was used to determine the significant differences in caleosin gene expression in each tissue. Rankings determined by Duncan’s test (p ≤ 0.05) are denoted by different letters, and are indicated on each bar in the graph. Bars not labeled are ranked as ‘a’. In stem tissue, the A, B, and R homeologues of Clo5 and Clo11 are all ranked as ‘ab’, and the A and B homeologues of Clo4 are ranked as ‘ab’.