Figure 1

Validation of the microarray. qPCR expression of selected genes: C3 (Complement component C3), IL-17D (Interleukin 17 D), LTBP-4 (Latent Transforming Growth Factor-β Binding Protein 4) and TRAF6 (tumor necrosis factor receptor-associated factor 6). Data are log2 transformed to facilitate the illustration of downregulated genes. All qPCR reactions were performed as technical triplicates and the expression level of analyzed genes was normalized using the 18S rRNA. Fold change units were then calculated by dividing the normalized expression values of hemocytes from infected clams by the normalized expression values of the controls. Each bar represents the mean and standard error of the biological replicates. Asterisks highlight the significant fold changes (t-test, p < 0.05). Microarray results (t-test, p < 0.01) are indicated in the boxes below the graph.